Peroxiredoxin 1 antibody (Middle Region)

Catalog No. ABIN3043903

Product Details anti-Peroxiredoxin 1 Antibody

Target: Peroxiredoxin 1 (PRDX1)

Binding Specificity: AA 100-128, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Peroxiredoxin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: Anti-Peroxiredoxin 1/PRDX1 Antibody Picoband®

Sequence: MNIPLVSDPK RTIAQDYGVL KADEGISFR

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Peroxiredoxin 1/PRDX1 Antibody Picoband® (ABIN3043903). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Peroxiredoxin 1, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Neumann, C. A., Krause, D. S., Carman, C. V., Das, S., Dubey, D. P., Abraham, J. L., Bronson, R. T., Fujiwara, Y., Orkin, S. H., Van Etten, R. A.Essential role for the peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumour suppression. Nature 424: 561-565, 2003. 2. Prosperi, M.-T., Apiou, F., Dutrillaux, B., Goubin, G. Organization and chromosomal assignment of two human PAG gene loci: PAGA encoding a functional gene and PAGB a processed pseudogene. Genomics 19: 236-241, 1994. 3. Yan, Y., Sabharwal, P., Rao, M., Sockanathan, S. The antioxidant enzyme Prdx1 controls neuronal differentiation by thiol-redox-dependent activation of GDE2. Cell 138: 1209-1221, 2009.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Peroxiredoxin 1

Target: Peroxiredoxin 1 (PRDX1)

Alternative Name: PRDX1 (PRDX1 Products)

Background:

Synonyms: Peroxiredoxin-1,1.11.1.15,Natural killer cell-enhancing factor A,NKEF-A,Proliferation-associated gene protein,PAG,Thioredoxin peroxidase 2,Thioredoxin-dependent peroxide reductase 2,PRDX1,PAGA, PAGB, TDPX2,

Tissue Specificity: Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

Background: PRDX1 (Peroxiredoxin 1), also called PRX1, PAGA or NKEFA, is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The PRDX1 gene is mapped on 1p34.1. Prdx1 was expressed in differentiating motor neuron cells in developing embryonic chicken and mouse spinal cords. mmunoprecipitation analysis showed that GDE2 interacted ly with PRDX1 in embryonic chicken spinal cord extracts and in transfected HEK293T cells. This protein may have a proliferative effect and play a role in cancer development or progression. In differentiating spinal cord, Prdx1 was required to activate Gde2 by reducing an intramolecular cystine bridge between the Gde2 N- and C-terminal domains. An intramolecular disulfide bond between the GDE2 N- and C-terminal domains inhibits GDE2 function, and that reduction of this cystine by PRDX1 activates GDE2 for the induction of motor neuron differentiation.

Sequence Similarities: Belongs to the AhpC/TSA family.

Molecular Weight: 24 kDa

Gene ID: 5052

UniProt: Q06830

Pathways: p53 Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events