PRNP antibody (AA 143-230)

Catalog No. ABIN3043910

The Rabbit Polyclonal anti-PRNP antibody has been validated for WB and IHC. It is suitable to detect PRNP in samples from Human, Rat and Mouse.

Quick Overview for PRNP antibody (AA 143-230) (ABIN3043910)

Target: PRNP (Prion Protein (PRNP))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRNP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PRNP Antibody

Binding Specificity: AA 143-230

Purpose: Anti-Prion protein PrP/PRNP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Prion protein PrP/PRNP Antibody Picoband® (ABIN3043910). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRNP recombinant protein (Position: S143-S230).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human
Western blot, 0.1-0.5 μg/mL, Rat, Human
1. Bounhar, Y., Zhang, Y., Goodyer, C. G., LeBlanc, A.Prion protein protects human neurons against Bax-mediated apoptosis.J. Biol. Chem. 276: 39145-39149, 2001. 2. Brown, P., Galvez, S., Goldfarb, L. G., Nieto, A., Cartier, L., Gibbs, C. J., Jr., Gajdusek, D. C.Familial Creutzfeldt-Jakob disease in Chile is associated with the codon 200 mutation of the PRNP amyloid precursor gene on chromosome 20.J. Neurol. Sci. 112: 65-67, 1992. 3. Puckett, C., Concannon, P., Casey, C., Hood, L.Genomic structure of the human prion protein gene.Am. J. Hum. Genet. 49: 320-329, 1991.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Alternative Name: PRNP

Background:

Synonyms: Major prion protein,PrP,ASCR,PrP27-30,PrP33-35C,CD230,PRNP,ALTPRP, PRIP, PRP,

Tissue Specificity: Expressed in breast, placenta, kidney, liver and pancreas. .

Background: PRNP (prion protein), also known as CD230 or PRP, is a protein that in humans is encoded by the PRNP gene. The major prion protein is expressed in the brain and several other tissues. Expression is most predominant in the nervous system but occurs in many other tissues throughout the body. Puckett et al. (1991) identified a RFLP with a high degree of heterozygosity in the 5-prime region of the PRNP gene, which might serve as a useful marker for the pter-p12 region of chromosome 20. PRNP is associated with a variety of cognitive deficiencies and neurodegenerative diseases such as Creutzfeldt-Jakob disease, bovine spongiform encephalopathy, and kuru. PRNP is highly conserved through mammals, lending credence to application of conclusions from test animals such as mice. Comparison between primates is especially similar, ranging from 92.9-99.6 % similarity in amino acid sequences.

Molecular Weight: 30 kDa

Gene ID: 5621

UniProt: P04156

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation