Retinoblastoma Binding Protein 4 antibody (C-Term)

Catalog No. ABIN3043920

This anti-Retinoblastoma Binding Protein 4 antibody is a Rabbit Polyclonal antibody detecting Retinoblastoma Binding Protein 4 in WB, IF, FACS, ICC and IHC (fro). Suitable for Human, Mouse and Rat.

Quick Overview for Retinoblastoma Binding Protein 4 antibody (C-Term) (ABIN3043920)

Target: Retinoblastoma Binding Protein 4 (RBBP4)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma Binding Protein 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Retinoblastoma Binding Protein 4 Antibody

Binding Specificity: AA 395-425, C-Term

Purpose: Anti-RbAp48/RBBP4 Antibody Picoband®

Sequence: EDNIMQVWQM AENIYNDEDP EGSVDPEGQG S

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RbAp48/RBBP4 Antibody Picoband® (ABIN3043920). Tested in Flow Cytometry, IF, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RbAp48, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. "Entrez Gene: RBBP4 retinoblastoma-binding protein 4". 2. Barak O, Lazzaro MA, Lane WS, Speicher DW, Picketts DJ, Shiekhattar R (November 2003). "Isolation of human NURF: a regulator of Engrailed gene expression". EMBO J 22 (22): 6089-100. 3. Qian YW, Wang YC, Hollingsworth RE Jr, Jones D, Ling N, Lee EY (September 1993). "A retinoblastoma-binding protein related to a negative regulator of Ras in yeast". Nature 364 (6438): 648-52.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Retinoblastoma Binding Protein 4

Target: Retinoblastoma Binding Protein 4 (RBBP4)

Alternative Name: RBBP4

Background:

Synonyms: Histone-binding protein RBBP4,Chromatin assembly factor 1 subunit C,CAF-1 subunit C,Chromatin assembly factor I p48 subunit,CAF-I 48 kDa subunit,CAF-I p48,Nucleosome-remodeling factor subunit RBAP48,Retinoblastoma-binding protein 4,RBBP-4,Retinoblastoma-binding protein p48,RBBP4,RBAP48,

Tissue Specificity: In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level). .

Background: Histone-binding protein RBBP4 (also known as RbAp48, or NURF55) is a protein that in humans is encoded by the RBBP4 gene. This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. And it is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind ly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 50-55 kDa

Gene ID: 5928

UniProt: Q09028

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Stem Cell Maintenance, Chromatin Binding, Protein targeting to Nucleus