SFTPA2 antibody (C-Term)

Catalog No. ABIN3043922

Product Details anti-SFTPA2 Antibody

Target: SFTPA2 (Surfactant Associated Protein A2 (SFTPA2))

Binding Specificity: AA 206-237, C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFTPA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Anti-SFTPA1/2 Antibody Picoband®

Sequence: VNYTNWYRGE PAGRGKEQCV EMYTDGQWND RN

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SFTPA1/2 Antibody Picoband® (ABIN3043922). Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SFTPA1/2, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat, -
Immunofluorescence, 5 μg/mL, Rat
1. Perez-Gil J, Weaver TE (June 2010). "Pulmonary surfactant pathophysiology: current models and open questions".Physiology (Bethesda) 25 (3): 132-41. 2. Phelps DS (2001). "Surfactant regulation of host defense function in the lung: a question of balance". Pediatr Pathol Mol Med 20 (4): 269-92.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SFTPA2

Target: SFTPA2 (Surfactant Associated Protein A2 (SFTPA2))

Alternative Name: SFTPA2 (SFTPA2 Products)

Background:

Synonyms: Pulmonary surfactant-associated protein A2,PSP-A,PSPA,SP-A,SP-A2,35 kDa pulmonary surfactant-associated protein,Alveolar proteinosis protein,Collectin-5,SFTPA2,COLEC5, PSAP, SFTP1, SFTPA, SFTPA2B,

Tissue Specificity: Expressed in B-cell lines and T-lymphocytes. .

Background: SFTPA1/2 is also known as SP-A. SFTPA1 encodes a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens. Mutations in this gene are associated with idiopathic pulmonary fibrosis. Alternate splicing results in multiple transcript variants. SFTPA2 is one of several genes encoding pulmonary-surfactant associated proteins (SFTPA) located on chromosome 10. Mutations in this gene and a highly similar gene located nearby, which affect the highly conserved carbohydrate recognition domain, are associated with idiopathic pulmonary fibrosis. The current version of the assembly displays only a single centromeric SFTPA gene pair rather than the two gene pairs shown in the previous assembly which were thought to have resulted from a duplication.

Sequence Similarities: Belongs to the SFTPA family.

Molecular Weight: 26 kDa

Gene ID: 653509, 729238