SIRT2 antibody (Middle Region)

Catalog No. ABIN3043924

This Rabbit Polyclonal antibody specifically detects SIRT2 in WB and IHC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for SIRT2 antibody (Middle Region) (ABIN3043924)

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SIRT2 Antibody

Binding Specificity: AA 165-187, Middle Region

Purpose: Anti-SIRT2 Antibody Picoband®

Sequence: YTQNIDTLER IAGLEQEDLV EAH

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SIRT2 Antibody Picoband® (ABIN3043924). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SIRT2, different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Dryden, S. C., Nahhas, F. A., Nowak, J. E., Goustin, A.-S., Tainsky, M. A. Role for humanSIRT2 NAD-dependent deacetylase activity in control of mitotic exit in the cell cycle. Molec. Cell Biol. 23: 3173-3185, 2003. 2. "Entrez Gene: SIRT2 sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)" 3. North, B. J., Marshall, B. L., Borra, M. T., Denu, J. M., Verdin, E. The human Sir2 ortholog, SIRT2, is an NAD(+)-dependent tubulin deacetylase. Molec. Cell 11: 437-444, 2003.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Alternative Name: SIRT2

Background:

Synonyms: NAD-dependent protein deacetylase sirtuin-2,3.5.1.-,Regulatory protein SIR2 homolog 2,SIR2-like protein 2,SIRT2,SIR2L, SIR2L2,

Tissue Specificity: Isoform 1 is expressed in heart, liver and skeletal muscle, weakly expressed in the cortex. Isoform 2 is strongly expressed in the cortex, weakly expressed in heart and liver. Weakly expressed in several malignancies including breast, liver, brain, kidney and prostate cancers compared to normal tissues. Weakly expressed in glioma cell lines compared to normal brain tissues (at protein level). Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. .

Background: SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT2 is mapped to 19q13.2. It is able to cause radioactivity to be transferred from (32P) NAD to bovine serum albumin (BSA). According to researches, SIRT2 can act as a bona fide tubulin deacetylase. SIRT2 was found to deacetylate lys40 of alpha-tubulin both in vitro and in vivo. SIRT2 is also a regulator of mitotic progression that acts downstream from CDC14B in a pathway regulating mitotic exit or subsequent cytokinesis. What's more, SIRT2 is an important regulator of programmed necrosis.

Sequence Similarities: Belongs to the sirtuin family. Class I subfamily.

Molecular Weight: 36 kDa

Gene ID: 22933