GLUT4 antibody (AA 333-509)

Catalog No. ABIN3043929

This anti-GLUT4 antibody is a Rabbit Polyclonal antibody detecting GLUT4 in WB and IHC. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 4+ publications.

Quick Overview for GLUT4 antibody (AA 333-509) (ABIN3043929)

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GLUT4 Antibody

Binding Specificity: AA 333-509

Purpose: Anti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband® (ABIN3043929). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GLUT4 recombinant protein (Position: N333-D509). Human GLUT4 shares 97% amino acid (aa) sequence identity with mouse GLUT4.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Rat, Human
1. James DE, Brown R, Navarro J, Pilch PF (May 1988). "Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein". Nature 333 (6169): 183-5. 2. James DE, Strube M, Mueckler M (March 1989). "Molecular cloning and characterization of an insulin-regulatable glucose transporter". Nature 338 (6210): 83-7. 3. Birnbaum MJ (April 1989). ""Identification of a novel gene encoding an insulin-responsive glucose transporter protein"". Cell 57 (2): 305-15.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-GLUT4 antibody (ABIN3043929)

Wei, An, Jin, Li, Xu: "Inhibition of insulin resistance by PGE1 via autophagy-dependent FGF21 pathway in diabetic nephropathy." in: Scientific reports, Vol. 8, Issue 1, pp. 9, (2018) (PubMed).

Li, Zhao, Qi, Wang, Zhang, Li, Qin: "lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma." in: International journal of oncology, Vol. 53, Issue 2, pp. 551-566, (2018) (PubMed).

Huang, Zhang, Sun, Xu, Yi: "Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 9651592, (2016) (PubMed).

Zhou, Wu, Chen, Wang, Wang: "AMP-activated protein kinase is required for the anti-adipogenic effects of alpha-linolenic acid." in: Nutrition & metabolism, Vol. 12, pp. 10, (2015) (PubMed).

Target Details for GLUT4

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Alternative Name: SLC2A4

Background:

Synonyms: Solute carrier family 2, facilitated glucose transporter member 4,Glucose transporter type 4, insulin-responsive,GLUT-4,SLC2A4,GLUT4,

Tissue Specificity: Skeletal and cardiac muscles, brown and white fat.

Background: GLUT4, also known as SLC2A4 or solute carrier family 2 (facilitated glucose transporter) member 4, is a protein that in humans is encoded by the GLUT4 gene. It is mapped to 17p13.1. This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).

Sequence Similarities: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

Molecular Weight: 55 kDa

Gene ID: 6517

UniProt: P14672

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Proton Transport, Brown Fat Cell Differentiation, Warburg Effect