STIM1 antibody (N-Term)

Catalog No. ABIN3043940

The Rabbit Polyclonal anti-STIM1 antibody has been validated for WB, IHC, IF, ICC and FACS. It is suitable to detect STIM1 in samples from Human, Rat and Mouse.

Quick Overview for STIM1 antibody (N-Term) (ABIN3043940)

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STIM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-STIM1 Antibody

Binding Specificity: AA 45-74, N-Term

Purpose: Anti-STIM1 Antibody Picoband®

Sequence: AAEFCRIDKP LCHSEDEKLS FEAVRNIHKL

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-STIM1 Antibody Picoband® (ABIN3043940). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human STIM1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Parker NJ, Begley CG, Smith PJ, Fox RM (Feb 1997). "Molecular cloning of a novel human gene (D11S4896E) at chromosomal region 11p15.5". Genomics 37 (2): 253-6. 2. Soboloff, Jonathan, Rothberg, B. S., Madesh, M., Gill, D. L. (September 2012)."STIM proteins: dynamic calcium signal transducers". Nat Rev Mol Cell Biol 13 (9): 549-65. 3. Williams RT, Manji SS, Parker NJ, Hancock MS, Van Stekelenburg L, Eid JP, Senior PV, Kazenwadel JS, Shandala T, Saint R, Smith PJ, Dziadek MA (Jul 2001)."Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins". Biochem J 357 (Pt 3): 673-85.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for STIM1

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Alternative Name: STIM1

Background:

Synonyms: Stromal interaction molecule 1,STIM1,GOK,

Tissue Specificity: Ubiquitously expressed in various human primary cells and tumor cell lines. .

Background: Stromal interaction molecule 1 is a protein that in humans is encoded by the STIM1 gene. STIM1 has a single transmembranedomain, and is localized to the endoplasmic reticulum, and to a lesser extent to the plasma membrane. This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants.

Sequence Similarities: Contains 1 EF-hand domain.

Molecular Weight: 97 kDa

Gene ID: 6786

UniProt: Q13586

Pathways: TCR Signaling, BCR Signaling