Versican antibody (N-Term)

Catalog No. ABIN3043957

This Rabbit Polyclonal antibody specifically detects Versican in WB and IHC. It exhibits reactivity toward Mouse, Human and Ratand has been mentioned in 1 publication.

Quick Overview for Versican antibody (N-Term) (ABIN3043957)

Target: Versican (Vcan)

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Versican antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Versican Antibody

Binding Specificity: AA 65-92, N-Term

Purpose: Anti-Versican/VCAN Antibody Picoband®

Sequence: KWSKIEVDKN GKDLKETTVL VAQNGNIK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Versican/VCAN Antibody Picoband® (ABIN3043957). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Versican, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Bode-Lesniewska, B., Dours-Zimmermann, M. T., Odermatt, B. F., Briner, J., Heitz, P. U., Zimmermann, D. R. Distribution of the large aggregating proteoglycan versican in adult human tissues. J. Histochem. Cytochem. 44: 303-312, 1996. 2. Dours-Zimmermann, M. T., Zimmermann, D. R. A novel glycosaminoglycan attachment domain identified in two alternative splice variants of human versican. J. Biol. Chem. 269: 32992-32998, 1994. 3. Naso, M. F., Morgan, J. L., Buchberg, A. M., Siracusa, L. D., Iozzo, R. V. Expression pattern and mapping of the murine versican gene (Cspg2) to chromosome 13. Genomics 29: 297-300, 1995.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Versican antibody (ABIN3043957)

Fujii, Karpova, Asagoe, Georgiev, Dummer, Urosevic-Maiwald: "Versican upregulation in Sézary cells alters growth, motility and resistance to chemotherapy." in: Leukemia, Vol. 29, Issue 10, pp. 2024-32, (2015) (PubMed).

Target Details for Versican

Target: Versican (Vcan)

Alternative Name: VCAN

Background:

Synonyms: Versican core protein,Chondroitin sulfate proteoglycan core protein 2,Chondroitin sulfate proteoglycan 2,Glial hyaluronate-binding protein,GHAP,Large fibroblast proteoglycan,PG-M,VCAN,CSPG2,

Tissue Specificity: Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.

Background: Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000 kDa. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.

Sequence Similarities: Belongs to the aggrecan/versican proteoglycan family.

Molecular Weight: 373 kDa

Gene ID: 1462

UniProt: P13611

Pathways: Glycosaminoglycan Metabolic Process