WASP antibody (C-Term)

Catalog No. ABIN3043961

The Rabbit Polyclonal anti-WASP antibody has been validated for WB, IHC and ICC. It is suitable to detect WASP in samples from Human, Mouse and Rat.

Quick Overview for WASP antibody (C-Term) (ABIN3043961)

Target: WASP (WAS) (Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WASP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-WASP Antibody

Binding Specificity: AA 129-156, C-Term

Purpose: Anti-WASP Antibody Picoband®

Sequence: ADEDEAQAFR ALVQEKIQKR NQRQSGDR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-WASP Antibody Picoband® (ABIN3043961). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human WASP, different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Jin, Y., Mazza, C., Christie, J., Giliani, S., Fiorini, M., Mella, P., Gandellini, F., Stewart, D., Zhu, Q., Nelson, D. L., Notarangelo, L. D., Ochs, H. D. (2004). "Mutations of the Wiskott-Aldrich Syndrome Protein (WASP): hotspots, effect on transcription, and translation and phenotype/genotype correlation". Blood104 (13): 4010-4019. 2. Radl, J., Dooren, L. H., Morell, A., Skvaril, F., Vossen, J. M., Uittenbogaart, C. H. (1976). "Immunoglobulins and transient paraproteins in sera of patients with the Wiskott-Aldrich syndrome: a follow-up study". Clinical and experimental immunology 25 (2): 256-263.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for WASP

Target: WASP (WAS) (Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS))

Alternative Name: WAS

Background:

Synonyms: Wiskott-Aldrich syndrome protein,WASp,WAS,IMD2,

Tissue Specificity: Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen. .

Background: The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, ly or inly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.

Sequence Similarities: Contains 1 CRIB domain.

Molecular Weight: 53 kDa

Gene ID: 7454

UniProt: P42768