Gelsolin antibody (C-Term)

Catalog No. ABIN3043978

This Rabbit Polyclonal antibody specifically detects Gelsolin in WB, IHC, ICC, FACS and IF. It exhibits reactivity toward Human, Mouse, Rat and Monkeyand has been mentioned in 1 publication.

Quick Overview for Gelsolin antibody (C-Term) (ABIN3043978)

Target: Gelsolin (GSN)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Gelsolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Gelsolin Antibody

Binding Specificity: AA 763-775, C-Term

Purpose: Anti-Gelsolin/GSN Antibody

Sequence: WDDDYWSVDP LDR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Gelsolin/GSN Antibody (ABIN3043978). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Gelsolin, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry , 0.5-1 μg/mL, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Gelsolin antibody (ABIN3043978)

Guo, Cui, Jiang, Zhang, Jiang: "Proteomics of acute heart failure in a rat post-myocardial infarction model." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1946-1956, (2018) (PubMed).

Target Details for Gelsolin

Target: Gelsolin (GSN)

Alternative Name: GSN

Background:

Synonyms: Gelsolin,AGEL,Actin-depolymerizing factor,ADF,Brevin,GSN,

Tissue Specificity: Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.

Background: Gelsolin also known as GNS is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. Gelsolin is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. Gelsolin is also known as brevin, or actin-depolymerizing factor, it is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. Gelsolin may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol.

Sequence Similarities: Belongs to the villin/gelsolin family.

Molecular Weight: 90 kDa

UniProt: P06396

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy