beta Arrestin 1 antibody (N-Term)

Catalog No. ABIN3043990

Product Details anti-beta Arrestin 1 Antibody

Target: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Binding Specificity: AA 82-103, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Arrestin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-beta Arrestin 1/ARRB1 Antibody Picoband®

Sequence: ANVQSFPPAP EDKKPLTRLQ ER

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-beta Arrestin 1/ARRB1 Antibody (ABIN3043990). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human beta Arrestin 1, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Buchanan, F. G., Gorden, D. L., Matta, P., Shi, Q., Matrisian, L. M., DuBois, R. N. Role of beta-arrestin 1 in the metastatic progression of colorectal cancer. Proc. Nat. Acad. Sci. 103: 1492-1497, 2006. 2. Nelson, C. D., Perry, S. J., Regier, D. S., Prescott, S. M., Topham, M. K., Lefkowitz, R. J.Targeting of diacylglycerol degradation to M1 muscarinic receptors by beta-arrestins. Science 315: 663-666, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for beta Arrestin 1

Target: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Alternative Name: ARRB1 (ARRB1 Products)

Background:

Synonyms: Beta-arrestin-1;Arrestin beta-1;ARRB1;ARR1;

Background: Arrestin, beta 1, also known as ARRB1, is a protein which in humans is encoded by the ARRB1 gene. Members of arrestin/beta-arrestin protein family are though to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. By fluorescence in situ hybridization, this gene is mapped to 11q13.4. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions.

Sequence Similarities: Belongs to the arrestin family.

Molecular Weight: 18 kDa

UniProt: P49407

Pathways: Positive Regulation of Peptide Hormone Secretion, Nuclear Hormone Receptor Binding, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction