FLI1 antibody (N-Term)

Catalog No. ABIN3043998

This anti-FLI1 antibody is a Rabbit Polyclonal antibody detecting FLI1 in WB and IHC. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for FLI1 antibody (N-Term) (ABIN3043998)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FLI1 Antibody

Binding Specificity: AA 54-73, N-Term

Purpose: Anti-FLI1 Antibody Picoband®

Sequence: QQEWINQPVR VNVKREYDHM

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-FLI1 Antibody (ABIN3043998). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human FLI1, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Donaldson, I. J., Chapman, M., Kinston, S., Landry, J. R., Knezevic, K., Piltz, S., Buckley, N., Green, A. R., Gottgens, B. Genome-wide identification of cis-regulatory sequences controlling blood and endothelial development. Hum. Molec. Genet. 14: 595-601, 2005. 2. Pimanda, J. E., Ottersbach, K., Knezevic, K., Kinston, S., Chan, W. Y. I., Wilson, N. K., Landry, J.-R., Wood, A. D., Kolb-Kokocinski, A., Green, A. R., Tannahill, D., Lacaud, G., Kouskoff, V., Gottgens, B. Gata2, Fli1, and Scl form a recursively wired gene-regulatory circuit during early hematopoietic development. Proc. Nat. Acad. Sci. 104: 17692-17697, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-FLI1 antibody (ABIN3043998)

Choi, El-Hamdi, McGregor: "Endothelial Cell Infection by Guinea Pig Cytomegalovirus Is a Lytic or Persistent Infection Depending on Tissue Origin but Requires Viral Pentamer Complex and pp65 Tegument Protein." in: Journal of virology, Vol. 96, Issue 17, pp. e0083122, (2022) (PubMed).

Target Details for FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Alternative Name: FLI1

Background:

Synonyms: Friend leukemia integration 1 transcription factor,Proto-oncogene Fli-1,Transcription factor ERGB,FLI1,

Background: Friend leukemia integration 1 transcription factor, also known as Transcription factor ERGB or Proto-oncogene Fli-1 is a protein that in humans is encoded by the human FLI1 gene. The fli1 protooncogene encodes a member of the ETS family of winged helix-turn-helix transcription factors. This gene is mapped to 11q24.3. Fli-1 is activated through retroviral insertional mutagenesis in 90 % of F-MuLV-induced erythroleukemias. The constitutive activation of fli-1 in erythroblasts leads to a dramatic shift in the Epo/Epo-R signal transduction pathway, blocking erythroid differentiation, activating the Ras pathway, and resulting in massive Epo-independent proliferation of erythroblasts. These results suggest that Fli-1 overexpression in erythroblasts alters their responsiveness to Epo and triggers abnormal proliferation by switching the signaling event (s) associated with terminal differentiation to proliferation.

Sequence Similarities: Belongs to the ETS family.

Molecular Weight: 53 kDa

UniProt: Q01543