NR4A3 antibody (C-Term)

Catalog No. ABIN3044046

The Rabbit Polyclonal anti-NR4A3 antibody (ABIN3044046) specifically detects NR4A3 in WB and IHC. The antibody is reactive with Human and Rat samples.

Quick Overview for NR4A3 antibody (C-Term) (ABIN3044046)

Target: NR4A3 (Nuclear Receptor Subfamily 4, Group A, Member 3 (NR4A3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR4A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NR4A3 Antibody

Binding Specificity: AA 612-631, C-Term

Purpose: Anti-Tyrosine-protein kinase Tec Tec Antibody

Sequence: FEDLLRTIDE LVECEETFGR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Tyrosine-protein kinase Tec Tec Antibody (ABIN3044046). Tested in IHC, WB applications. This antibody reacts with Human, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Tec, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Thimerosal, 0.01 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NR4A3

Target: NR4A3 (Nuclear Receptor Subfamily 4, Group A, Member 3 (NR4A3))

Alternative Name: TEC

Background:

Synonyms: Tyrosine-protein kinase Tec,2.7.10.2,TEC,PSCTK4,

Tissue Specificity: Expressed in a wide range of cells, including hematopoietic cell lines like myeloid, B-, and T-cell lineages.

Background: TEC (TEC Protein Tyrosine Kinase), is an enzyme that in humans is encoded by the TEC gene. The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. By fluorescence in situ hybridization, Sato et al. (1994) mapped the gene to 4p12, the same location reported for TXK. Mouse Tec is a non-receptor type protein-tyrosine kinase that is highly expressed in many hematopoietic cell lines. Hantschel et al. (2007) identified TEC kinase and BTK kinase as major binders of the tyrosine kinase inhibitor dasatinib, which is used for treatment of BCR/ABL-positive CML.

Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.

Molecular Weight: 58-60 kDa

UniProt: P42680

Pathways: Fc-epsilon Receptor Signaling Pathway, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway