EIF6 antibody (Middle Region)

Catalog No. ABIN3044059

Product Details anti-EIF6 Antibody

Target: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Binding Specificity: AA 82-96, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 6(EIF6) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Sequence: QHIRNSLPDT VQIRR

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 6(EIF6) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: eukaryotic translation initiation factor 6
Protein Name: Eukaryotic translation initiation factor 6

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human EIF6(82-96aa QHIRNSLPDTVQIRR), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EIF6

Target: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Alternative Name: EIF6 (EIF6 Products)

Synonyms: CAB antibody, EIF3A antibody, ITGB4BP antibody, b(2)gcn antibody, eIF-6 antibody, p27(BBP) antibody, p27BBP antibody, 1110004P11 antibody, AA408895 antibody, Itgb4bp antibody, imc-415 antibody, MGC56562 antibody, itgb4bp4 antibody, zgc:56562 antibody, wu:fc28a04 antibody, wu:ft88f05 antibody, itgb4bp antibody, eukaryotic translation initiation factor 6 antibody, Eukaryotic translation initiation factor 6 antibody, eukaryotic translation initiation factor 6 S homeolog antibody, translation initiation factor eIF6 antibody, EIF6 antibody, Eif6 antibody, eif6 antibody, eif-6 antibody, eif6.S antibody, tif6 antibody

Background: EIF6(Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al.(1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al.(2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al.(2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50 % reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

Synonyms: b(2)gcn antibody|B(2)GCN homolog antibody|B4 integrin interactor antibody|Binding protein of beta-4 integrin antibody|CAB antibody|eIF-6 antibody|EIF3A antibody|EIF6 antibody|Eukaryotic translation initiation factor 3A antibody|Eukaryotic translation initiation factor 6 antibody|IF6_HUMAN antibody|ITGB4BP antibody|OK/SW-cl.27 antibody|p27 beta 4 integrin binding protein antibody|p27(BBP) antibody|p27BBP antibody|RP4-614O4.1 antibody

UniProt: P56537

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly