Somatostatin antibody (C-Term)

Catalog No. ABIN3044066

Product Details anti-Somatostatin Antibody

Target: Somatostatin (SST)

Binding Specificity: AA 89-107, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Somatostatin antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Somatostatin(SST) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequence: SANSNPAMAP RERKAGCKN

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Somatostatin(SST) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: somatostatin
Protein Name: Somatostatin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Somatostatin(89-107aa SANSNPAMAPRERKAGCKN), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for SST is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-Somatostatin antibody (ABIN3044066)

Mao, Lu, Wang, Tian, Huang, Feng, Zhang, Chang: "Role of PI3K p110β in the differentiation of human embryonic stem cells into islet-like cells." in: Biochemical and biophysical research communications, Vol. 488, Issue 1, pp. 109-115, (2017) (PubMed).

Wang, Zhou, Zhang, Wu, Zhang, Zhang: "Identification and localization of gastrointestinal hormones in the skin of the bullfrog Rana catesbeiana during periods of activity and hibernation." in: Acta histochemica, Vol. 116, Issue 8, pp. 1418-26, (2014) (PubMed).

Chen, He, Peng, Liu, Jin, Cao, Wang, Xiao: "An immunohistochemical study of somatostatin in the stomach and the small intestine of the African ostrich (Struthio camelus)." in: Tissue & cell, Vol. 45, Issue 6, pp. 363-6, (2013) (PubMed).

Jiang, Deng, Duan, Chen, Xiang, Lu, Ma: "Somatostatin receptors SSTR2 and SSTR5 are expressed in the human thoracic duct." in: Lymphology, Vol. 44, Issue 1, pp. 21-8, (2011) (PubMed).

Zong, Chen, Zhang, Zou: "Effects of intra-gastric beta-casomorphin-7 on somatostatin and gastrin gene expression in rat gastric mucosa." in: World journal of gastroenterology, Vol. 13, Issue 14, pp. 2094-9, (2007) (PubMed).

Target Details for Somatostatin

Target: Somatostatin (SST)

Alternative Name: SST (SST Products)

Synonyms: SMST antibody, SOM antibody, SRIF antibody, SS antibody, Smst antibody, SS-14 antibody, SS-28 antibody, SS1 antibody, som antibody, smst antibody, PPS antibody, SOM14 antibody, SST antibody, sst antibody, somatostatin antibody, somatostatin S homeolog antibody, SST antibody, Sst antibody, sst.S antibody, sst antibody, sst.2 antibody

Target Type: Chemical

Background: SST(Somatostatin), also known as SMST, is a peptide hormone. Naylor et al.(1983) assigned the somatostatin gene to chromosome 3 by analyzing somatic cell hybrids with a polymorphic gene probe. Yacubova and Komuro(2002) examined the effects of somatostatin in cerebellar granule cells of early postnatal mice, because these cells express all 5 types of somatostatin receptors before the initiation of their migration. Saito et al.(2005) found that somatostatin modulated the proteolytic degradation of beta-amyloid catalyzed by neprilysin both in vitro and in vivo.

Synonyms: Growth hormone release inhibiting factor antibody|SMST antibody|Somatostatin antibody|Somatostatin 28 antibody|SST antibody

UniProt: P61278