RIPK2 antibody (C-Term)

Catalog No. ABIN3044089

This Rabbit Polyclonal antibody specifically detects RIPK2 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RIPK2 antibody (C-Term) (ABIN3044089)

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RIPK2 Antibody

Binding Specificity: AA 495-514, C-Term

Purpose: Anti-RIP2/RIPK2 Antibody Picoband®

Sequence: DIQGEEFAKV IVQKLKDNKQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-RIP2/RIPK2 Antibody (ABIN3044089). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RIP2, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. McCarthy, J. V., Ni, J., Dixit, V. M. RIP2 is a novel NF-kappa-B-activating and cell death-inducing kinase. J. Biol. Chem. 273: 16968-16975, 1998.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RIPK2

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Alternative Name: RIPK2

Background:

Synonyms: Receptor-interacting serine/threonine-protein kinase 2;2.7.11.1;CARD-containing interleukin-1 beta-converting enzyme-associated kinase;CARD-containing IL-1 beta ICE-kinase;RIP-like-interacting CLARP kinase;Receptor-interacting protein 2;RIP-2;Tyrosine-protein kinase RIPK2;2.7.10.2;RIPK2;CARDIAK, RICK, RIP2;UNQ277/PRO314/PRO34092;

Tissue Specificity: Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.

Background: RIPK2 (Receptor-interacting serine/threonine-protein kinase 2), also known as CARD3, CARDIAK, RICK, RIP2, is an enzyme that in humans is encoded by the RIPK2 gene. It has 540-amino acid protein in length. Northern blot analysis revealed that RICK is expressed in various human tissues as 2.5- and 1.8-kb mRNAs that differ due to alternative polyadenylation. RICK is a novel kinase that may regulate apoptosis induced by the FAS receptor pathway. This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappa B and inducer of apoptosis in response to various stimuli, CARDIAK (CARD-containing ICE-associated kinase) specifically interacted with the CARD of ICE/caspase-1, and this interaction correlated with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 subunit.

Sequence Similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Molecular Weight: 61 kDa

UniProt: O43353

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades