MAG antibody (N-Term)

Catalog No. ABIN3044143

Product Details anti-MAG Antibody

Target: MAG (Myelin Associated Glycoprotein (MAG))

Binding Specificity: AA 114-132, N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Myelin-associated glycoprotein(MAG) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: KYYFRGDLGG YNQYTFSEH

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Myelin-associated glycoprotein(MAG) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: myelin associated glycoprotein
Protein Name: Myelin-associated glycoprotein

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MAG(114-132aa KYYFRGDLGGYNQYTFSEH), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for MAG

Target: MAG (Myelin Associated Glycoprotein (MAG))

Alternative Name: MAG (MAG Products)

Synonyms: GMA antibody, S-MAG antibody, SIGLEC-4A antibody, SIGLEC4A antibody, siglec-4 antibody, MAG antibody, Gma antibody, siglec-4a antibody, myelin associated glycoprotein antibody, myelin-associated glycoprotein antibody, MAG antibody, mag antibody, Mag antibody

Background: MAG(Myelin-associated glycoprotein),also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor.The MAG gene is mapped on 19q13.12. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons were rendered MAG-sensitive by expression of NgR. MAG binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with MAG prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney(HEK) cells expressing the NOGO receptor, p75(NTR) was required for MAG-induced intracellular calcium elevation.

Synonyms: GMA antibody|MAG antibody|MAG_HUMAN antibody|Myelin associated glycoprotein antibody|Myelin-associated glycoprotein antibody|S MAG antibody|Siglec 4a antibody|Siglec-4a antibody|SIGLEC4A antibody

UniProt: P20916

Pathways: Neurotrophin Signaling Pathway