SSR3 antibody (N-Term)

Catalog No. ABIN3044181

Product Details anti-SSR3 Antibody

Target: SSR3 (SSRg) (Signal Sequence Receptor gamma (SSRg))

Binding Specificity: AA 8-23, N-Term

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSR3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purpose: Anti-SSR3 Antibody Picoband®

Sequence: KQQSEEDLLL QDFSRN

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SSR3 Antibody (ABIN3044181). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SSR3, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human, Mouse, Rat
1. Gross, M. B.Personal Communication.Baltimore, Md. 6/20/2012. 2. Hartmann, E., Gorlich, D., Kostka, S., Otto, A., Kraft, R., Knespel, S., Burger, E., Rapoport, T. A., Prehn, S.A tetrameric complex of membrane proteins in the endoplasmic reticulum.Europ. J. Biochem. 214: 375-381, 1993.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SSR3

Target: SSR3 (SSRg) (Signal Sequence Receptor gamma (SSRg))

Alternative Name: SSR3 (SSRg Products)

Background:

Synonyms: Translocon-associated protein subunit gamma,TRAP-gamma,Signal sequence receptor subunit gamma,SSR-gamma,SSR3,TRAPG,

Tissue Specificity: Highly expressed in the brain, lung, kidney and heart. Expressed at low levels in the liver and spleen. .

Background: SSR3 (signal sequence receptor, gamma) is also known as TRAPG. By immunoaffinity chromatography, biochemical purification, peptide sequence analysis, and screening of a rat liver cDNA library, Hartmann et al. (1993) obtained a cDNA encoding Ssr3, which they termed Trap-gamma. The deduced 185-amino acid protein spans the membrane 4 times and is expressed as a 20-kD protein. Gross (2012)mapped the SSR3 gene to chromosome 3q25.31 based on an alignment of the SSR3 sequence with the genomic sequence (GRCh37). Hartmann et al. (1993)found that Ssr3 was associated in equal amounts with Ssr1, Ssr2, and Ssr4 in canine pancreatic microsomes.

Molecular Weight: 21 kDa