HOXA3 antibody (C-Term)

Catalog No. ABIN3044212

This Rabbit Polyclonal antibody specifically detects HOXA3 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HOXA3 antibody (C-Term) (ABIN3044212)

Target: HOXA3 (Homeobox A3 (HOXA3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOXA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HOXA3 Antibody

Binding Specificity: AA 427-443, C-Term

Purpose: Anti-Homeobox protein Hox-A3 HOXA3 Antibody Picoband®

Sequence: HHPSQGRIQE APKLTHL

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Homeobox protein Hox-A3 HOXA3 Antibody (ABIN3044212). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HOXA3, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Greer, J. M., Puetz, J., Thomas, K. R., Capecchi, M. R. Maintenance of functional equivalence during paralogous Hox gene evolution. Nature 403: 661-665, 2000. 2. McAlpine PJ, Shows TB (Aug 1990). "Nomenclature for human homeobox genes". 3. Han L, Witmer PD, Casey E, Valle D, Sukumar S (2007). "DNA methylation regulates MicroRNA expression".

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HOXA3

Target: HOXA3 (Homeobox A3 (HOXA3))

Alternative Name: HOXA3

Background:

Synonyms: Homeobox protein Hox-A3,Homeobox protein Hox-1E,HOXA3,HOX1E,

Tissue Specificity: Widely expressed.

Background: HOXA3 (HOMEOBOX A3) is a protein that in humans is encoded by the HOXA3 gene, which is also part of the A cluster on chromosome 7. HOXA3 encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Its cytogenetic location is 7q15.2. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. HOXA3 protein is able to complement Hoxd3 deficiency when expressed in the context of the Hoxd3 allele.

Sequence Similarities: Belongs to the Antp homeobox family.

Molecular Weight: 46 kDa

UniProt: O43365