BUB3 antibody (Middle Region)

Catalog No. ABIN3044239

Product Details anti-BUB3 Antibody

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Binding Specificity: AA 76-90, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Mitotic checkpoint protein BUB3(BUB3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequence: DHQLKMHDLN TDQEN

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Mitotic checkpoint protein BUB3(BUB3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: budding uninhibited by benzimidazoles 3 homolog(yeast)
Protein Name: Mitotic checkpoint protein BUB3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Bub3(76-90aa DHQLKMHDLNTDQEN), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Alternative Name: BUB3 (BUB3 Products)

Synonyms: BUB3L antibody, hBUB3 antibody, Aa2-050 antibody, xbub3 antibody, AU019800 antibody, AU021329 antibody, AU043350 antibody, AW146323 antibody, C78067 antibody, BUB3, mitotic checkpoint protein antibody, BUB3 mitotic checkpoint protein antibody, BUB3 mitotic checkpoint protein L homeolog antibody, BUB3 antibody, Bub3 antibody, bub3.L antibody

Background: BUB3(BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint(SAC), though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation. Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.

Synonyms: Aa2-050 antibody|AU019800 antibody|AU021329 antibody|AU043350 antibody|AW146323 antibody|BUB 3 antibody|BUB 3L antibody|BUB3(budding uninhibited by benzimidazoles 3, yeast) homolog antibody|bub3 antibody|BUB3 budding uninhibited by benzimidazoles 3 homolog antibody|BUB3_HUMAN antibody|BUB3L antibody|Budding uninhibited by benomyl antibody|Budding uninhibited by benzimidazoles 3 antibody|Budding uninhibited by benzimidazoles 3 homolog(S. cerevisiae) antibody|Budding uninhibited by benzimidazoles 3 homolog(yeast) antibody|Budding uninhibited by benzimidazoles 3 homolog antibody|Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody|C78067 antibody|hBUB3 antibody|Mitotic checkpoint component antibody|Mitotic checkpoint protein bub3 antibody|OTTHUMP00000020679 antibody|OTTHUMP00000020680 antibody|WD repeat type I transmembrane protein A72.5 antibody

UniProt: O43684

Pathways: Maintenance of Protein Location