Netrin 1 antibody (N-Term)

Catalog No. ABIN3044248

This Rabbit Polyclonal antibody specifically detects Netrin 1 in WB. It exhibits reactivity toward Human, Mouse, Rat and has been mentioned in 2+ publications.

Quick Overview for Netrin 1 antibody (N-Term) (ABIN3044248)

Target: Netrin 1 (NTN1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Netrin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Netrin 1 Antibody

Binding Specificity: AA 32-51, N-Term

Purpose: Anti-Netrin 1/NTN1 Antibody Picoband®

Sequence: AGQAAQPDPC SDENGHPRRC

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Netrin 1/NTN1 Antibody (ABIN3044248). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Netrin 1, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Colon-Ramos, D. A., Margeta, M. A., Shen, K. Glia promote local synaptogenesis through UNC-6 (netrin) signaling in C. elegans. Science 318: 103-106, 2007. 2. Corset, V., Nguyen-Ba-Charvet, K. T., Forcet, C., Moyse, E., Chedotal, A., Mehlen, P. Netrin-1-mediated axon outgrowth and cAMP production requires interaction with adenosine A2b receptor. Nature 407: 747-750, 2000. 3. Powell AW, Sassa T, Wu Y, Tessier-Lavigne M, Polleux F (May 2008). Ghosh, Anirvan. ed. "Topography of thalamic projections requires attractive and repulsive functions of Netrin-1 in the ventral telencephalon."

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Netrin 1 antibody (ABIN3044248)

Qian-hua, Shao-ping, Jian-wen, Yun, Li: "Reduced expression of netrin-1 is associated with fetal growth restriction." in: Molecular and cellular biochemistry, Vol. 350, Issue 1-2, pp. 81-7, (2011) (PubMed).

Zhong, Zou, Zhao, Hu, Xie: "Effect of different concentrations of neogenin on proliferation, apoptosis and related proliferative factors in human trophoblasts." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 30, Issue 4, pp. 500-4, (2010) (PubMed).

Target Details for Netrin 1

Target: Netrin 1 (NTN1)

Alternative Name: NTN1

Background:

Synonyms: Netrin-1,Epididymis tissue protein Li 131P,NTN1,NTN1L,

Tissue Specificity: Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level). .

Background: Netrin-1 (NTN1), also known as NTN1L, is a diffusible protein made by floor plate cells. Netrin-1 is found in the floor plate and neuroepithelial cells of the ventral region of the spinal cord, as well as other locations in the nervous system including the somatic mesoderm, pancreas and cardiac muscle. The human NTN1 gene is mapped to chromosome 17p13-p12 by FISH. Netrin-1 can attract spinal commissural axons and repel trochlear axons in vitro. And netrin-1 expression confers a selective advantage for tumor cell survival. The binding of netrin-1 to DCC appears to depend on the presence of a coreceptor or accessory proteins. Adenosine A2b receptor is actually a netrin-1 receptor and induces cAMP accumulation on binding netrin-1, and that netrin-1-dependent outgrowth of dorsal spinal cord axons ly involves A2b. Netrin-1 can promote intestinal tumor development, probably by regulating cell survival. Thus, a netrin-1 receptor or receptors function as conditional tumor suppressors. Expression of Unc5b triggered endothelial cell repulsion in response to netrin-1 in vitro, whereas a truncated Unc5b lacking the intracellular signaling domain failed to induce repulsion.

Sequence Similarities: Contains 3 laminin EGF-like domains.

Molecular Weight: 8 kDa

UniProt: O95631

Pathways: Regulation of Cell Size