Myoglobin antibody (C-Term)

Catalog No. ABIN3044306

The Rabbit Polyclonal anti-Myoglobin antibody has been validated for WB and IHC. It is suitable to detect Myoglobin in samples from Human, Rat and Mouse. There are 2+ publications available.

Quick Overview for Myoglobin antibody (C-Term) (ABIN3044306)

Target: Myoglobin (MB)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myoglobin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Myoglobin Antibody

Binding Specificity: AA 138-154, C-Term

Purpose: Anti-Myoglobin/MB Antibody Picoband®

Sequence: LFRNDIAAKY KELGFQG

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Rabbit IgG polyclonal antibody for Myoglobin(MB) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: myoglobin
Protein Name: myoglobin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Myoglobin, identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
1. Boyer, S. H., Fainer, D. C., Naughton, M. A. Myoglobin inherited structural variation in man. Science 140: 1228-1231, 1963. 2. Boulton, F. E., Huntsman, R. G., Lorkin, P. A., Lehmann, H. Abnormal human myoglobin: 53(D4) glutamic acid lysine. Nature 223: 832-833, 1969. 3. Jeffreys, A. J., Wilson, V., Blanchetot, A., Weller, P., Geurts van Kessel, A., Spurr, N., Solomon, E., Goodfellow, P. The human myoglobin gene: a third dispersed globin locus in the human genome. Nucleic Acids Res. 12: 3235-3243, 1984.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Myoglobin antibody (ABIN3044306)

Xu, He, Zhang, Fan, Zhao, Xu, Nie, Li, Huang, Lu, Xu: "Synthesis and in vitro evaluation of a hyaluronic acid-quantum dots-melphalan conjugate." in: Carbohydrate polymers, Vol. 121, pp. 132-9, (2015) (PubMed).

Peng, Yanjiao, Ai-guo, Pengtao, Jianhua, Ju, Hongsheng, Xichen: "A fine balance between CCNL1 and TIMP1 contributes to the development of breast cancer cells." in: Biochemical and biophysical research communications, Vol. 409, Issue 2, pp. 344-9, (2011) (PubMed).

Target Details for Myoglobin

Target: Myoglobin (MB)

Alternative Name: Myoglobin

Background:

Synonyms: Myoglobin,Mb,

Background: Myoglobin (MB) also known as PVALB, is a single-chain globular protein of 153 or 154 amino acids, containing a heme (iron-containing porphyrin) prosthetic group in the center around which the remaining apoprotein folds. It is a member of the globin superfamily and is expressed in skeletal and cardiac muscles. This gene is mapped to chromosome 22q11-q13. Myoglobin is released from damaged muscle tissue (rhabdomyolysis), which has very high concentrations of myoglobin. The released myoglobin is filtered by the kidneys but is toxic to the renal tubular epithelium and so may cause acute renal failure.

Molecular Weight: 17 kDa

UniProt: P04247

Pathways: Brown Fat Cell Differentiation