Integrin alpha 1 antibody (C-Term)

Catalog No. ABIN3044325

This anti-Integrin alpha 1 antibody is a Rabbit Polyclonal antibody detecting Integrin alpha 1 in WB and IHC. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for Integrin alpha 1 antibody (C-Term) (ABIN3044325)

Target: Integrin alpha 1 (ITGA1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin alpha 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Integrin alpha 1 Antibody

Binding Specificity: AA 1121-1134, C-Term

Purpose: Anti-Integrin alpha 1/ITGA1 Antibody Picoband®

Sequence: SNQKRELAIQ ISKD

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Integrin alpha 1/ITGA1 Antibody (ABIN3044325). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha 1, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Douville, P., Seldin, M. F., Carbonetto, S. : Genetic mapping of the integrin alpha-1 gene (Vla1) to mouse chromosome 13. Genomics 14: 503-505, 1992. 2. Lee HJ, Kim SY, Koh JM, Bok J, Kim KJ, Kim KS, Park MH, Shin HD, Park BL, Kim TH, Hong JM, Park EK, Kim DJ, Oh B, Kimm K, Kim GS, Lee JY. Polymorphisms and haplotypes of integrinalpha1 (ITGA1) are associated with bone mineral absorbance and fracture risk in postmenopausal Koreans.Bone. 2007 Dec,41(6):979-86. Epub 2007 Sep 5. 3. Ekholm, E., Hankenson, K. D., Uusitalo, H., Hiltunen, A., Gardner, H., Heino, J., Penttinen, R. : Diminished callus size and cartilage synthesis in alpha-1 beta-1 integrin-deficient mice during bone fracture healing. Am. J. Path. 160: 1779-1785, 2002.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Integrin alpha 1 antibody (ABIN3044325)

Zhang, Ren, Luo, Fan, Liu, Liang, Ma, Yu, Bai: "A small physiological electric field mediated responses of extravillous trophoblasts derived from HTR8/SVneo cells: involvement of activation of focal adhesion kinase signaling." in: PLoS ONE, Vol. 9, Issue 3, pp. e92252, (2014) (PubMed).

Target Details for Integrin alpha 1

Target: Integrin alpha 1 (ITGA1)

Alternative Name: ITGA1

Background:

Synonyms: Integrin alpha-1,CD49 antigen-like family member A,Laminin and collagen receptor,VLA-1,CD49a,ITGA1,

Tissue Specificity: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .

Background: Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Sequence Similarities: Belongs to the integrin alpha chain family.

Molecular Weight: 190-200 kDa

UniProt: P56199

Pathways: EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex