N-Cadherin antibody (Middle Region)

Catalog No. ABIN3044370

Product Details anti-N-Cadherin Antibody

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Binding Specificity: AA 701-714, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Anti-N-Cadherin-2 CDH2 CD325-Antibody Picoband®

Sequence: CQCDSNGDCT DVDR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-N-Cadherin-2 CDH2 -Antibody (ABIN3044370). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human N Cadherin, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Miyatani, S., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Takeichi, M. : Genomic structure and chromosomal mapping of the mouse N-cadherin gene. Proc. Nat. Acad. Sci. 89: 8443-8447, 1992. 2. Walsh, F. S., Barton, C. H., Putt, W., Moore, S. E., Kelsell, D., Spurr, N., Goodfellow, P. N. : N-cadherin gene maps to human chromosome 18 and is not linked to the E-cadherin gene. J. Neurochem. 55: 805-812, 1990. 3. Togashi, H., Abe, K., Mizoguchi, A., Takaoka, K., Chisaka, O., Takeichi, M. : Cadherin regulates dendritic spine morphogenesis. Neuron 35: 77-89, 2002.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-N-Cadherin antibody (ABIN3044370)

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Shen, Cai, Liu, Li, Gan, Li, Wang, Guo, Zhou, Lu, Sun, Li: "Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy." in: Journal of hematology & oncology, Vol. 11, Issue 1, pp. 95, (2018) (PubMed).

Pan, An, Meng, Li, Ren, Guan: "MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/β-catenin pathway." in: Experimental and therapeutic medicine, Vol. 14, Issue 5, pp. 4663-4670, (2017) (PubMed).

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Shan, Lv, Zhao, Liu, Sun, Xi, Xiao, Li: "Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 10, pp. 12357-67, (2016) (PubMed).

Lin, Sun, Wang, Zheng, Zhang, Zheng: "Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition." in: Frontiers in pharmacology, Vol. 6, pp. 320, (2016) (PubMed).

Tong, Sun, Wang, Suo: "Sorcin Enhances Metastasis and Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer." in: Cell biochemistry and biophysics, (2015) (PubMed).

Liu, Liu, Jiang, Peng, Ma, Lu: "SOX9 Overexpression Promotes Glioma Metastasis via Wnt/?-Catenin Signaling." in: Cell biochemistry and biophysics, (2015) (PubMed).

Sun, Hu, Zheng, Bagnoli, Guo, Rupaimoole, Rodriguez-Aguayo, Lopez-Berestein, Ji, Chen, Sood, Mezzanzanica, Liu, Sun, Zhang: "MiR-506 inhibits multiple targets in the epithelial-to-mesenchymal transition network and is associated with good prognosis in epithelial ovarian cancer." in: The Journal of pathology, Vol. 235, Issue 1, pp. 25-36, (2015) (PubMed).

Su, Li: "RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10760-7, (2015) (PubMed).

Yang, Hui, Wang, Yang, Jiang: "Overexpression of SOX2 promotes migration, invasion, and epithelial-mesenchymal transition through the Wnt/?-catenin pathway in laryngeal cancer Hep-2 cells." in: Tumour biology, Vol. 35, Issue 8, pp. 7965-73, (2014) (PubMed).

Kudo-Sakamoto, Akazawa, Ito, Takano, Yano, Yabumoto, Naito, Oka, Lee, Sakata, Suzuki, Saido, Komuro: "Calpain-dependent cleavage of N-cadherin is involved in the progression of post-myocardial infarction remodeling." in: The Journal of biological chemistry, Vol. 289, Issue 28, pp. 19408-19, (2014) (PubMed).

Wang, Li, Zhou, Sun, Wang, Cheng, Ma, Liu, Liu, Wang, Liang, Wu: "Intervention effects of ganoderma lucidum spores on epileptiform discharge hippocampal neurons and expression of neurotrophin-4 and N-cadherin." in: PLoS ONE, Vol. 8, Issue 4, pp. e61687, (2013) (PubMed).

Qiao, Johnson, Gao et al.: "Epithelial-mesenchymal transition in oral squamous cell carcinoma triggered by transforming growth factor-beta1 is Snail family-dependent and correlates with matrix metalloproteinase-2 and -9 ..." in: International journal of oncology, Vol. 37, Issue 3, pp. 663-8, (2010) (PubMed).

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: CDH2 (CDH2 Products)

Background:

Synonyms: Cadherin-2,CDw325,Neural cadherin,N-cadherin,CD325,CDH2,CDHN, NCAD,

Background: N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96 % identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Molecular Weight: 140 kDa

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane