DLD antibody (C-Term)

Catalog No. ABIN3044405

The Rabbit Polyclonal anti-DLD antibody has been validated for WB, IHC, IF, ICC and IHC (fro). It is suitable to detect DLD in samples from Human, Mouse and Rat.

Quick Overview for DLD antibody (C-Term) (ABIN3044405)

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DLD Antibody

Binding Specificity: AA 492-509, C-Term

Purpose: Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband®

Sequence: EAFREANLAA SFGKSINF

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Lipoamide Dehydrogenase/DLD Antibody (ABIN3044405). Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DLD, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Mouse
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Feigenbaum, A. S., Robinson, B. H.The structure of the human dihydrolipoamide dehydrogenase gene (DLD) and its upstream elements.Genomics 17: 376-381, 1993. 2. Sakata, Y., Owada, Y., Sato, K., Kojima, K., Hisanaga, K., Shinka, T., Suzuki, Y., Aoki, Y., Satoh, J., Kondo, H., Matsubara, Y., Kure, S.Structure and expression of the glycine cleavage system in rat central nervous system.Molec. Brain Res. 94: 119-130, 2001. 3. Scherer, S. W., Otulakowski, G., Robinson, B. H., Tsui, L.-C.Localization of the human dihydrolipoamide dehydrogenase gene (DLD) to 7q31-q32.Cytogenet. Cell Genet. 56: 176-177, 1991.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Thimerosal, 0.01 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DLD

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Alternative Name: DLD

Background:

Synonyms: Dihydrolipoyl dehydrogenase, mitochondrial,1.8.1.4,Dihydrolipoamide dehydrogenase,Glycine cleavage system L protein,DLD,GCSL, LAD, PHE3,

Tissue Specificity: Placenta.

Background: DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.

Sequence Similarities: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Molecular Weight: 54 kDa

UniProt: P09622

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis