IGFBP3 antibody (Middle Region)

Catalog No. ABIN3044421

Cited in 2+ publications. The Rabbit Polyclonal anti-IGFBP3 antibody (ABIN3044421) specifically detects IGFBP3 in WB. The antibody is reactive with Human and Rat samples.

Quick Overview for IGFBP3 antibody (Middle Region) (ABIN3044421)

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGFBP3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IGFBP3 Antibody

Binding Specificity: AA 182-198, Middle Region

Purpose: Anti-IGFBP3 Antibody

Sequence: IKKGHAKDSQ RYKVDYE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-IGFBP3 Antibody (ABIN3044421). Tested in WB applications. This antibody reacts with Human, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human IGFBP-3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-IGFBP3 antibody (ABIN3044421)

Pu, Wen, Liu, Zheng, Xiao, Xu, Wang, Li, Zhang: "shRNA constructs targeting IGFBP-3 alleviate age related erectile dysfunction in the rat." in: The Journal of urology, Vol. 192, Issue 3, pp. 990-6, (2014) (PubMed).

Pu, Zheng, Zhang, Xiao, Xu, Liu, Wang, Wen, Zhou, Wu: "Higher expression of mRNA and protein of insulin-like growth factor binding protein-3 in old rat penile tissues: implications for erectile dysfunction." in: The journal of sexual medicine, Vol. 8, Issue 8, pp. 2181-90, (2011) (PubMed).

Target Details for IGFBP3

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Alternative Name: IGFBP3

Background:

Synonyms: Insulin-like growth factor-binding protein 3,IBP-3,IGF-binding protein 3,IGFBP-3,IGFBP3,IBP3,

Tissue Specificity: Expressed by most tissues. Present in plasma.

Background: IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Sequence Similarities: Contains 1 IGFBP N-terminal domain.

Molecular Weight: 40 kDa

UniProt: P17936

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding