HMMR antibody (C-Term)

Catalog No. ABIN3044455

Product Details anti-HMMR Antibody

Target: HMMR (Hyaluronan-Mediated Motility Receptor (RHAMM) (HMMR))

Binding Specificity: AA 706-724, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMMR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-CD168/HMMR Antibody Picoband®

Sequence: KEGNTNCYRA PMECQESWK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-/HMMR Antibody (ABIN3044455). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD168.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Entwistle, J., Zhang, S., Yang, B., Wong, C., Li, Q., Hall, C. L., Jingbo, A., Mowat, M., Greenberg, A. H., Turley, E. A.Characterization of the murine gene encoding the hyaluronan receptor RHAMM.Gene 163: 233-238, 1995. 2. Hall, C., Yang, B., Yang, X., Zhang, S., Turley, M., Samuel, S., Lange, L. A., Wang, C., Curpen, G. D., Savani, R. C., Greenberg, A. H., Turley, E. A.Overexpression of the hyaluronan receptor RHAMM is transforming and is also required for H-ras transformation.Cell 82: 19-28, 1995. 3. Savani, R. C., Wang, C., Yang, B. H., Zhang, S. W., Kinsella, M. G., Wight, T. N., Stern, R., Nance, D. M., Turley, E. A.Migration of bovine aortic smooth muscle cells after wounding injury: the role of hyaluronan and RHAMM.J. Clin. Invest. 95: 1158-1168, 1995.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-HMMR antibody (ABIN3044455)

Ozbilgin, Boz, Tuğyan, Inan, Vatansever: "RHAMM Expression in the Rat Endometrium during the Estrous Cycle and following Implantation." in: Journal of reproduction & infertility, Vol. 13, Issue 3, pp. 131-7, (2013) (PubMed).

Target Details for HMMR

Target: HMMR (Hyaluronan-Mediated Motility Receptor (RHAMM) (HMMR))

Alternative Name: HMMR (HMMR Products)

Background:

Synonyms: Hyaluronan mediated motility receptor,Intracellular hyaluronic acid-binding protein,Receptor for hyaluronan-mediated motility,CD168,HMMR,IHABP, RHAMM,

Background: HMMR, Hyaluronan-mediated motility receptor, is a protein which in humans is encoded by the HMMR gene. RHAMM was originally discovered as a soluble protein that altered migratory cell behavior and bound to hyaluronan, HMMR is less well studied than the main hyaluronan (HA) receptor, CD44. In contrast to CD44 and other cell-surface receptors which contain the classical membrane spanning domain and signal sequence for secretion from the endoplasmic reticulum/Golgi complex, HMMR does not contain a membrane spanning domain nor does the mRNA transcript contain a signal sequence. HMMR normally is localized inside the cell and is only release by certain, poorly defined stimuli. The transport of HMMR to the extracellular space still is unclear but may involve transport channels or proteins, flippase activity, or exocytosis. Intracellularly, HMMR associates with microtubules and, working with BRCA1 and BARD1, plays a role in the regulation of mitosis. Extracellularly, HMMR associates with CD44, and upon binding to HA, activates intracellular signaling pathways. Variants of HMMR caused by alternative splicing have been observed, but not thoroughly studied.

Molecular Weight: 84 kDa

UniProt: O75330

Pathways: Glycosaminoglycan Metabolic Process