Ceruloplasmin antibody (AA 20-258)

Catalog No. ABIN3044508

This anti-Ceruloplasmin antibody is a Rabbit Polyclonal antibody detecting Ceruloplasmin in WB and IHC. Suitable for Rat and Mouse.

Quick Overview for Ceruloplasmin antibody (AA 20-258) (ABIN3044508)

Target: Ceruloplasmin (CP) (Ceruloplasmin (Ferroxidase) (CP))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ceruloplasmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Ceruloplasmin Antibody

Binding Specificity: AA 20-258

Purpose: Anti-Ceruloplasmin/CP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Ceruloplasmin(CP) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: ceruloplasmin (ferroxidase)
Protein Name: Ceruloplasmin

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Ceruloplasmin recombinant protein (Position: R20-M258). Mouse Ceruloplasmin shares 80.8% and 91.2% amino acid (aa) sequence identity with human and rat Ceruloplasmin, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
1. Koschinsky ML, Funk WD, van Oost BA, MacGillivray RT (Jul 1986)."Complete cDNA sequence of human preceruloplasmin". Proceedings of the National Academy of Sciences of the United States of America 83 (14): 5086-90. 2. Royle NJ, Irwin DM, Koschinsky ML, MacGillivray RT, Hamerton JL (May 1987). "Human genes encoding prothrombin and ceruloplasmin map to 11p11-q12 and 3q21-24, respectively". Somatic Cell and Molecular Genetics 13(3): 285-92. 3. Takahashi N, Ortel TL, Putnam FW (Jan 1984). "Single-chain structure of human ceruloplasmin: the complete amino acid sequence of the whole molecule". Proceedings of the National Academy of Sciences of the United States of America 81 (2): 390-4.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Ceruloplasmin

Target: Ceruloplasmin (CP) (Ceruloplasmin (Ferroxidase) (CP))

Alternative Name: Cp

Background:

Synonyms: Ceruloplasmin;1.16.3.1;Ferroxidase;Cp;

Tissue Specificity: Expressed in many tissues, including liver, eye and brain. .

Background: Ceruloplasmin  (or caeruloplasmin) is a ferroxidase enzyme that in humans is encoded by the CP gene. It is mapped to 3q23-q25. The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe (II)transferrin to Fe (III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene.

Molecular Weight: 130 kDa

Gene ID: 12870

UniProt: Q61147

Pathways: Transition Metal Ion Homeostasis