MGA (AA 2376-2415), (C-Term) antibody

Catalog No. ABIN3044528

The Rabbit Polyclonal anti- antibody has been validated for WB. It is suitable to detect in samples from Human.

Quick Overview for MGA (AA 2376-2415), (C-Term) antibody (ABIN3044528)

Target: MGA

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: AA 2376-2415, C-Term

Purpose: Anti-MGA Antibody Picoband®

Sequence: QKEAEAFAYY RRTHTANERR RRGEMRDLFE KLKITLGLLH

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MGA Antibody Picoband® (ABIN3044528). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MGA, different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Hurlin, P. J., Steingrimsson, E., Copeland, N. G., Jenkins, N. A., Eisenman, R. N. Mga, a dual-specificity transcription factor that interacts with Max and contains a T-domain DNA-binding motif. EMBO J. 18: 7019-7028, 1999. Note: Erratum: EMBO J. 19: 3841 only, 2000. 2. McIver KS, Myles RL (March 2002). "Two DNA-binding domains of Mga are required for virulence gene activation in the group A streptococcus". Mol. Microbiol. 43 (6): 1591-601.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details

Target: MGA

Background:

Synonyms: MAX gene-associated protein;MAX dimerization protein 5;MGA;KIAA0518, MAD5;

Tissue Specificity: Expressed in all tissues tested with high levels in thymus.

Background: Mga is a DNA-binding protein that activates the expression of several important virulence genes in Streptococcus pyogenes  (group A Streptococcus, GAS) in response to changing environmental conditions. It had been found that the mouse transcription factor Max interacted with Mga. Coimmunoprecipitation analysis confirmed that Max and Mga interacted in transfected HEK293 cells. EMSA revealed that Mga required Max for binding to the E-box sequence CACGTG. The isolated T-box of Mga bound the brachyury T-box-binding site in the absence of Max. Mga repressed transcription of a reporter driven from a T-box-binding site, but coexpression of Mga with Max caused transcriptional activation from the T-box-binding site. Expression of Mga with Max, but not Mga alone, activated transcription from a reporter containing the E-box site.

Molecular Weight: 334 kDa

Gene ID: 23269