Prokineticin 1 antibody (AA 20-105)

Catalog No. ABIN3044536

This Rabbit Polyclonal antibody specifically detects Prokineticin 1 in WB and ELISA. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Prokineticin 1 antibody (AA 20-105) (ABIN3044536)

Target: Prokineticin 1 (Prok1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prokineticin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Prokineticin 1 Antibody

Binding Specificity: AA 20-105

Purpose: Anti-Prokineticin 1/PROK1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Prokineticin 1/PROK1 Antibody Picoband® (ABIN3044536). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Prokineticin 1 recombinant protein (Position: A20-F105). Human Prokineticin 1 shares 88.4% and 91.9% amino acid (aa) sequence identity with mouse and rat Prokineticin 1, respectively.

Isotype: IgG

Application Details

Application Notes: ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Human
1. LeCouter, J., Kowalski, J., Foster, J., Hass, P., Zhang, Z., Dillard-Telm, L., Frantz, G., Rangell, L., DeGuzman, L., Keller, G.-A., Peale, F., Gurney, P., Hillan, K. J., Ferrara, N. Identification of an angiogenic mitogen selective for endocrine gland endothelium. Nature 412: 877-884, 2001. 2. Li, M., Bullock, C. M., Knauer, D. J., Ehlert, F. J., Zhou, Q. Y. Identification of two prokineticin cDNAs: recombinant proteins potently contract gastrointestinal smooth muscle. Molec. Pharm. 59: 692-698, 2001.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Prokineticin 1 antibody (ABIN3044536)

Yu, Chen, Jiang: "Administration of pigment epithelium-derived factor delivered by adeno-associated virus inhibits blood-retinal barrier breakdown in diabetic rats." in: Molecular vision, Vol. 16, pp. 2384-94, (2011) (PubMed).

Target Details for Prokineticin 1

Target: Prokineticin 1 (Prok1)

Alternative Name: PROK1

Background:

Synonyms: Prokineticin-1,Endocrine-gland-derived vascular endothelial growth factor,EG-VEGF,Mambakine,PROK1,UNQ600/PRO1186,

Tissue Specificity: Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta. .

Background: Prokineticin-1 is also known as EG-VEGF. The International Radiation Hybrid Mapping Consortium mapped the PROK1 gene to chromosome 1. The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner.

Molecular Weight: 20 kDa

Gene ID: 84432

UniProt: P58294