SKAP1 antibody (Middle Region)

Catalog No. ABIN3044545

This Rabbit Polyclonal antibody specifically detects SKAP1 in WB and IHC. It exhibits reactivity toward Human and Rat.

Quick Overview for SKAP1 antibody (Middle Region) (ABIN3044545)

Target: SKAP1 (Src Kinase Associated phosphoprotein 1 (SKAP1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SKAP1 Antibody

Binding Specificity: AA 163-189, Middle Region

Purpose: Anti-SKAP55/SKAP1 Antibody Picoband®

Sequence: RMAPHLRRDS KKESCFELTS QDRRSYE

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SKAP55/SKAP1 Antibody Picoband® (ABIN3044545). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SKAP55, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, Human
Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: SKAP1 src kinase associated phosphoprotein 1". 2. Marie-Cardine A, Bruyns E, Eckerskorn C, Kirchgessner H, Meuer SC, Schraven B (July 1997). "Molecular cloning of SKAP55, a novel protein that associates with the protein tyrosine kinase p59fyn in human T-lymphocytes". J Biol Chem 272 (26): 16077-80. 3. Wang H, Moon EY, Azouz A, et al. (2003). "SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates". Nat. Immunol. 4 (4): 366-74.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SKAP1

Target: SKAP1 (Src Kinase Associated phosphoprotein 1 (SKAP1))

Alternative Name: SKAP1

Background:

Synonyms: Src kinase-associated phosphoprotein 1,Src family-associated phosphoprotein 1,Src kinase-associated phosphoprotein of 55 kDa,SKAP-55,pp55,SKAP1,SCAP1, SKAP55,

Tissue Specificity: Highly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level). .

Background: Src kinase-associated phosphoprotein 1 is an adapter protein that in humans is encoded by the SKAP1 gene. This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.

Molecular Weight: 60 kDa

Gene ID: 8631