SMURF1 antibody (Middle Region)

Catalog No. ABIN3044547

The Rabbit Polyclonal anti-SMURF1 antibody (ABIN3044547) specifically detects SMURF1 in WB, IHC, IF, FACS and ICC. The antibody is reactive with Human and Rat samples.

Quick Overview for SMURF1 antibody (Middle Region) (ABIN3044547)

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMURF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-SMURF1 Antibody

Binding Specificity: AA 326-351, Middle Region

Purpose: Anti-SMURF1 Antibody

Sequence: DHNNRTTQFT DPRLHHIMNH QCQLKE

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SMURF1 Antibody (ABIN3044547). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SMURF1, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SMURF1

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Alternative Name: SMURF1

Background:

Synonyms: E3 ubiquitin-protein ligase SMURF1,hSMURF1,6.3.2.-,SMAD ubiquitination regulatory factor 1,SMAD-specific E3 ubiquitin-protein ligase 1,SMURF1,KIAA1625,

Tissue Specificity: Expressed in melanocytes (PubMed:23999003). .

Background: E3 ubiquitin-protein ligase SMURF1 is an enzyme that in humans is encoded by the SMURF1 gene. It is mapped to 7q22.1. This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 86 kDa

Gene ID: 57154

Pathways: Regulation of Cell Size