SMYD3 antibody (C-Term)

Catalog No. ABIN3044548

Product Details anti-SMYD3 Antibody

Target: SMYD3 (SET and MYND Domain Containing 3 (SMYD3))

Binding Specificity: AA 388-428, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMYD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-SMYD3 Antibody Picoband®

Sequence: QAMKNLRLAF DIMRVTHGRE HSLIEDLILL LEECDANIRA S

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SMYD3 Antibody Picoband® (ABIN3044548). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SMYD3, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Hamamoto, R., Furukawa, Y., Morita, M., Iimura, Y., Silva, F. P., Li, M., Yagyu, R., Nakamura, Y. SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells. Nature Cell Biol. 6: 731-740, 2004. 2. Mazur, P. K., Reynoird, N., Khatri, P., Jansen, P. W. T. C., Wilkinson, A. W., Liu, S., Barbash, O., Van Aller, G. S., Huddleston, M., Dhanak, D., Tummino, P. J., Kruger, R. G., Garcia, B. A., Butte, A. J., Vermeulen, M., Sage, J., Gozani, O. SMYD3 links lysine methylation of MAP3K2 to Ras-driven cancer. Nature 510: 283-287, 2014. 3. Tsuge, M., Hamamoto, R., Silva, F. P., Ohnishi, Y., Chayama, K., Kamatani, N., Furukawa, Y., Nakamura, Y. A variable number of tandem repeats polymorphism in an E2F-1 binding element in the 5-prime flanking region of SMYD3 is a risk factor for human cancers. Nature Genet. 37: 1104-1107, 2005.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SMYD3

Target: SMYD3 (SET and MYND Domain Containing 3 (SMYD3))

Alternative Name: SMYD3 (SMYD3 Products)

Background:

Synonyms: Histone-lysine N-methyltransferase SMYD3,2.1.1.43,SET and MYND domain-containing protein 3,Zinc finger MYND domain-containing protein 1,SMYD3,ZMYND1, ZNFN3A1,

Tissue Specificity: Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas. .

Background: SET and MYND domain-containing protein 3 is a protein that in humans is encoded by the SMYD3 gene. The International Radiation Hybrid Mapping Consortium mapped the SMYD3 gene to chromosome 1. This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 55 kDa

Gene ID: 64754

UniProt: Q9H7B4