TPP1 antibody (Middle Region)

Catalog No. ABIN3044554

This Rabbit Polyclonal antibody specifically detects TPP1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for TPP1 antibody (Middle Region) (ABIN3044554)

Target: TPP1 (Tripeptidyl Peptidase I (TPP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TPP1 Antibody

Binding Specificity: AA 227-261, Middle Region

Purpose: Anti-TPP1 Antibody Picoband®

Sequence: CAQFLEQYFH DSDLAQFMRL FGGNFAHQAS VARVV

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TPP1 Antibody Picoband® (ABIN3044554). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human TPP1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: TPP1 tripeptidyl peptidase I". 2. Liu CG, Sleat DE, Donnelly RJ, Lobel P (Jun 1998). "Structural organization and sequence of CLN2, the defective gene in classical late infantile neuronal ceroid lipofuscinosis". Genomics 50 (2): 206-12.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TPP1

Target: TPP1 (Tripeptidyl Peptidase I (TPP1))

Alternative Name: TPP1

Background:

Synonyms: Tripeptidyl-peptidase 1,TPP-1,3.4.14.9,Cell growth-inhibiting gene 1 protein,Lysosomal pepstatin-insensitive protease,LPIC,Tripeptidyl aminopeptidase,Tripeptidyl-peptidase I,TPP-I,TPP1,CLN2,GIG1, UNQ267/PRO304,

Tissue Specificity: Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues.

Background: Tripeptidyl-peptidase 1, also known as Lysosomal pepstatin-insensitive protease, is an enzyme that in humans is encoded by the TPP1 gene. This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.

Molecular Weight: 39 kDa

Gene ID: 1200

UniProt: O14773

Pathways: Cell Division Cycle, ER-Nucleus Signaling