TPP1 antibody (Middle Region)

Catalog No. ABIN3044554

Product Details anti-TPP1 Antibody

Target: TPP1 (Tripeptidyl Peptidase I (TPP1))

Binding Specificity: AA 227-261, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-TPP1 Antibody Picoband®

Sequence: CAQFLEQYFH DSDLAQFMRL FGGNFAHQAS VARVV

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TPP1 Antibody Picoband® (ABIN3044554). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human TPP1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: TPP1 tripeptidyl peptidase I". 2. Liu CG, Sleat DE, Donnelly RJ, Lobel P (Jun 1998). "Structural organization and sequence of CLN2, the defective gene in classical late infantile neuronal ceroid lipofuscinosis". Genomics 50 (2): 206-12.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TPP1

Target: TPP1 (Tripeptidyl Peptidase I (TPP1))

Alternative Name: TPP1 (TPP1 Products)

Background:

Synonyms: Tripeptidyl-peptidase 1,TPP-1,3.4.14.9,Cell growth-inhibiting gene 1 protein,Lysosomal pepstatin-insensitive protease,LPIC,Tripeptidyl aminopeptidase,Tripeptidyl-peptidase I,TPP-I,TPP1,CLN2,GIG1, UNQ267/PRO304,

Tissue Specificity: Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues.

Background: Tripeptidyl-peptidase 1, also known as Lysosomal pepstatin-insensitive protease, is an enzyme that in humans is encoded by the TPP1 gene. This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.

Molecular Weight: 39 kDa

Gene ID: 1200

UniProt: O14773

Pathways: Cell Division Cycle, ER-Nucleus Signaling