VRK1 antibody (C-Term)

Catalog No. ABIN3044560

This Rabbit Polyclonal antibody specifically detects VRK1 in WB. It exhibits reactivity toward Human and Rat.

Quick Overview for VRK1 antibody (C-Term) (ABIN3044560)

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VRK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VRK1 Antibody

Binding Specificity: AA 292-329, C-Term

Purpose: Anti-VRK1 Antibody Picoband®

Sequence: EKNKPGEIAK YMETVKLLDY TEKPLYENLR DILLQGLK

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-VRK1 Antibody Picoband® (ABIN3044560). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human VRK1, different from the related mouse sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. "Entrez Gene: VRK1 vaccinia related kinase 1". 2. Nezu J, Oku A, Jones MH, Shimane M (Feb 1998). "Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to vaccinia virus B1R kinase". Genomics 45 (2): 327-31.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for VRK1

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Alternative Name: VRK1

Background:

Synonyms: Serine/threonine-protein kinase VRK1,2.7.11.1,Vaccinia-related kinase 1,VRK1,

Tissue Specificity: Widely expressed. Highly expressed in fetal liver, testis and thymus. .

Background: Serine/threonine-protein kinase VRK1 is an enzyme that in humans is encoded by the VRK1 gene. This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. It is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 Molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.

Molecular Weight: 55 kDa

Gene ID: 7443

UniProt: Q99986