YY1 antibody (Middle Region)

Catalog No. ABIN3044562

This anti-YY1 antibody is a Rabbit Polyclonal antibody detecting YY1 in WB, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for YY1 antibody (Middle Region) (ABIN3044562)

Target: YY1 (YY1 Transcription Factor (YY1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YY1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-YY1 Antibody

Binding Specificity: AA 206-241, Middle Region

Purpose: Anti-YY1 Antibody Picoband®

Sequence: EQKQVQIKTL EGEFSVTMWS SDEKKDIDHE TVVEEQ

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-YY1 Antibody Picoband® (ABIN3044562). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human YY1, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Shi Y, Seto E, Chang LS, Shenk T (Oct 1991). "Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein". Cell 67 (2): 377-88. 2. Thiaville MM, Kim J (2011). "Oncogenic potential of yin yang 1 mediated through control of imprinted genes". Critical Reviews in Oncogenesis 16 (3-4): 199-209. 3. Zhu W, Lossie AC, Camper SA, Gumucio DL (Apr 1994). "Chromosomal localization of the transcription factor YY1 in the mouse and human". Mammalian Genome 5 (4): 234-6.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for YY1

Target: YY1 (YY1 Transcription Factor (YY1))

Alternative Name: YY1

Background:

Synonyms: Transcriptional repressor protein YY1,Delta transcription factor,INO80 complex subunit S,NF-E1,Yin and yang 1,YY-1,YY1,INO80S,

Tissue Specificity: Widely expressed. .

Background: YY1 (Yin Yang 1) is a transcriptional repressor protein in humans that is encoded by the YY1 gene. YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

Molecular Weight: 65 kDa

Gene ID: 7528

UniProt: P25490