beta Actin antibody (N-Term)

Catalog No. ABIN3044573

Product Details anti-beta Actin Antibody

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Binding Specificity: AA 2-19, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Actin, cytoplasmic 1(ACTB) detection. Tested with WB in Human,Mouse,Rat.

Sequence: DDDIAALVVD NGSGMCKA

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Actin, cytoplasmic 1(ACTB) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: actin, beta
Protein Name: Actin, cytoplasmic 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin(2-19aaDDDIAALVVDNGSGMCKA), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-beta Actin antibody (ABIN3044573)

Zhai, Liu, Wang, Zhang, Li, Guo: "Picroside II protects the blood-brain barrier by inhibiting the oxidative signaling pathway in cerebral ischemia-reperfusion injury." in: PLoS ONE, Vol. 12, Issue 4, pp. e0174414, (2017) (PubMed).

Chang, Zhang, Lihua, Feng: "T3 inhibits the calcification of vascular smooth muscle cells and the potential mechanism." in: American journal of translational research, Vol. 8, Issue 11, pp. 4694-4704, (2016) (PubMed).

Wang, Qi, Li, Wu, Wang, Wang, Ning: "Inhibitory effects of Chikusetsusaponin IVa on lipopolysaccharide-induced pro-inflammatory responses in THP-1 cells." in: International journal of immunopathology and pharmacology, Vol. 28, Issue 3, pp. 308-17, (2015) (PubMed).

Zuo, Liu, Zhang, Wu, Guo, Liao: "Development of trastuzumab-resistant human gastric carcinoma cell lines and mechanisms of drug resistance." in: Scientific reports, Vol. 5, pp. 11634, (2015) (PubMed).

Gong, Wang, Yuan, Li, Gu, Zhao, Zhang, Jia, Feng, Liu: "Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines of Scutellaria barbata D. Don in Lewis-Bearing C57BL/6 Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 630760, (2015) (PubMed).

Wang, Wu, Wang, Tian, Chen, Li: "The biophysical property of A549 cells transferred by VEGF-D." in: Scanning, Vol. 36, Issue 2, pp. 202-8, (2015) (PubMed).

Lee, Lee, Huo, Wang, Huang, Lin, Chuang, Lee: "Diabetes enhances the intrahepatic vascular response to endothelin-1 in cirrhotic rats: association with the ETA receptor and pERK up-regulation." in: Liver international : official journal of the International Association for the Study of the Liver, Vol. 35, Issue 3, pp. 704-12, (2015) (PubMed).

Qiao, Wei, Dai, Zhou, Yin, Li, Xiao, Guo, Lu: "UCP2-related mitochondrial pathway participates in oroxylin A-induced apoptosis in human colon cancer cells." in: Journal of cellular physiology, Vol. 230, Issue 5, pp. 1054-63, (2015) (PubMed).

Wang, Wei, Wang, Du, Zhang, Zhou: "Cellular activation, expression analysis and functional characterization of grass carp I?B?: evidence for its involvement in fish NF-?B signaling pathway." in: Fish & shellfish immunology, Vol. 42, Issue 2, pp. 408-12, (2015) (PubMed).

Zheng, Ding, Ni, Song, Huang, Wang: "MiR-142 acts as a tumor suppressor in osteosarcoma cell lines by targeting Rac1." in: Oncology reports, Vol. 33, Issue 3, pp. 1291-9, (2015) (PubMed).

Shi, Yang, Mao, Wu, Ren, Yang, Yin, Wei, Li, Wang: "Nutlin-3-induced redistribution of chromatin-bound IFI16 in human hepatocellular carcinoma cells in vitro is associated with p53 activation." in: Acta pharmacologica Sinica, Vol. 36, Issue 2, pp. 252-8, (2015) (PubMed).

Pan, Chou, Sun: "P2X7 R-mediated Ca(2+) -independent d-serine release via pannexin-1 of the P2X7 R-pannexin-1 complex in astrocytes." in: Glia, Vol. 63, Issue 5, pp. 877-93, (2015) (PubMed).

Gong, Liu, Nie, Li, Xie, Liu, Liu, Mei, Li: "MiR-21/RASA1 axis affects malignancy of colon cancer cells via RAS pathways." in: World journal of gastroenterology, Vol. 21, Issue 5, pp. 1488-97, (2015) (PubMed).

Wang, Li, Tang, Yang, Wang, Liu, Yang, Yang: "Overexpression of Tiam1 promotes the progression of laryngeal squamous cell carcinoma." in: Oncology reports, Vol. 33, Issue 4, pp. 1807-14, (2015) (PubMed).

Fu, Lin, Liang, Hu: "Effects of recombinant baculovirus AcMNPV-BmK IT on the formation of early cables and nuclear polymerization of actin in Sf9 cells." in: Cytotechnology, (2015) (PubMed).

Wang, Gai, Xu, Zheng, Lv, Li, Liu: "Shikonin protects chondrocytes from interleukin-1beta-induced apoptosis by regulating PI3K/Akt signaling pathway." in: International journal of clinical and experimental pathology, Vol. 8, Issue 1, pp. 298-308, (2015) (PubMed).

Rong, Ding, Zhang, Guo: "Neuregulin1? improves cognitive dysfunction and up-regulates expression of p-ERK1/2 in rats with chronic omethoate poisoning." in: Behavioral and brain functions : BBF, Vol. 11, pp. 5, (2015) (PubMed).

Yang, Yan, Liu, Wang, Hu: "Protective effects of acteoside against X?ray?induced damage in human skin fibroblasts." in: Molecular medicine reports, Vol. 12, Issue 2, pp. 2301-6, (2015) (PubMed).

Shi, Wu, Wang, Zhou, Yan, Chen, Gu, Cai: "Liquiritigenin Potentiates the Inhibitory Effects of Cisplatin on Invasion and Metastasis Via Downregulation MMP-2/9 and PI3 K/AKT Signaling Pathway in B16F10 Melanoma Cells and Mice Model." in: Nutrition and cancer, Vol. 67, Issue 5, pp. 761-70, (2015) (PubMed).

Zhao, Wang, Meng, Ji, Xu, Chen, Fan, Yu, Yao, Dong: "MicroRNA-27a-3p Inhibits Melanogenesis in Mouse Skin Melanocytes by Targeting Wnt3a." in: International journal of molecular sciences, Vol. 16, Issue 5, pp. 10921-33, (2015) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB (ACTB Products)

Synonyms: BRWS1 antibody, PS1TP5BP1 antibody, ACTA1 antibody, Actx antibody, E430023M04Rik antibody, beta-actin antibody, ps1tp5bp1 antibody, act-2 antibody, actb antibody, B-actin antibody, LOC100174883 antibody, LOC100304816 antibody, A antibody, ACT5C antibody, Ac5C antibody, Act antibody, Act-5C antibody, Act5 antibody, Act5c antibody, Actin antibody, Actin/BAP47 antibody, Actin5C antibody, BAP47 antibody, Bap47 antibody, CG4027 antibody, Dmel\\CG4027 antibody, M32055 antibody, T11 antibody, act antibody, act 5C antibody, act5C antibody, actin antibody, actin5C antibody, anon-EST:fe2D2 antibody, beta-actin/Bap47 antibody, cyt5C antibody, l(1)G0009 antibody, l(1)G0010 antibody, l(1)G0025 antibody, l(1)G0079 antibody, l(1)G0117 antibody, l(1)G0177 antibody, l(1)G0245 antibody, l(1)G0330 antibody, l(1)G0420 antibody, l(1)G0486 antibody, Bact antibody, ACT-5 antibody, ACTB antibody, B-ACTZF antibody, actba antibody, bact antibody, bactin1 antibody, bactzf antibody, wu:fd18f05 antibody, A26C1B antibody, POTE2alpha antibody, POTEACTIN antibody, Beta-actin antibody, actin beta antibody, actin, beta antibody, cytoplasmic actin OlCA1 antibody, beta-actin antibody, Actin 5C antibody, actin gamma 1 antibody, actin, beta 1 antibody, actin, beta L homeolog antibody, beta actin antibody, POTE ankyrin domain family member F antibody, actin, beta like 2 antibody, actin, cytoplasmic 1 antibody, ACTB antibody, Actb antibody, actb antibody, LOC100174883 antibody, LOC100304816 antibody, Act5C antibody, ACTG1 antibody, actb1 antibody, actb.L antibody, LOC100136352 antibody, POTEF antibody, ACTBL2 antibody, LOC109073280 antibody

Background: Beta-Actin(ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1, is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al.(1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al.(2006) found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al.(2006) concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.

Synonyms: A26C1A antibody|A26C1B antibody|ACTB antibody|ACTB_HUMAN antibody|Actin cytoplasmic 1 antibody|Actx antibody|b-actin antibody|beta cytoskeletal actin antibody|Beta-actin antibody|E430023M04Rik antibody|MGC128179 antibody|PS1TP5 binding protein 1 antibody|PS1TP5BP1 antibody

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction