MHC Class II (RT1Bu) antibody (FITC)

Catalog No. ABIN305163

This anti- antibody is a Mouse Monoclonal antibody detecting in FACS. Suitable for Rat and Mouse.

Quick Overview for MHC Class II (RT1Bu) antibody (FITC) (ABIN305163)

Target: MHC Class II (RT1Bu)

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS)

Clone: OX-3

Product Details

Specificity: Recognizes a polymorphic determinant of rat Ia antigen (RT1. Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. Is useful for distinguishing Ia positive cells from different rat strains, e. g. for recognizing cells of donor origin in bone marrow reconstituted radiation chimaeras. MRC OX-3 Reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Recognizes Ia antigens on B-cells, dendritic cells and certain epithelial cells. This product is routinely tested in flow cytometry on Lewis rat splenocytes.

Purification: Purified

Immunogen: Rat thymocyte membrane glycoproteins.

Type of Immunogen: Membrane extract

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:1)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: MHC Class II (RT1Bu)

Alternative Name: MHC Class II RT-1Bu