MHC Class II RT-1B antibody (FITC)
Quick Overview for MHC Class II RT-1B antibody (FITC) (ABIN305180)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. The clone does not react with the Rat BDIX strain. This product is routinely tested in flow cytometry on rat splenocytes.
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Purification
- Affinity purified
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Immunogen
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Rat thymocyte membrane glycoproteins.
Type of Immunogen: Membrane extract -
Isotype
- IgG1
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Application Notes
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Approved: Flo (1:1 - 1:10)
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Comment
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Target Species of Antibody: Rat
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, pH 7.4, 0.09 % sodium azide, 1 % BSA.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- MHC Class II RT-1B
Target
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