CD3 antibody

Catalog No. ABIN305216

The Rat Monoclonal anti-CD3 antibody has been validated for FACS, IHC and IHC (fro). It is suitable to detect CD3 in samples from Mouse.

Quick Overview for CD3 antibody (ABIN305216)

Target: CD3

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: KT3

Product Details anti-CD3 Antibody

Specificity: Recognizes the mouse CD3 antigen, which is expressed by mature T cells. The antibody may be used to trigger proliferation and cytotoxicity of CD3 positive cells. NB. For optimal staining incubations should be performed at room temperature.

Purification: Affinity purified

Immunogen:

Type of Immunogen: Native protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:100), IHC, IHC-Fr

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway