CD3 antibody

Catalog No. ABIN305267

The Mouse Monoclonal anti-CD3 antibody has been validated for FACS, IP, IHC, IHC (fro) and IHC (p). It is suitable to detect CD3 in samples from Rat.

Quick Overview for CD3 antibody (ABIN305267)

Target: CD3

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1F4

Product Details anti-CD3 Antibody

Specificity: Recognizes rat CD3, a 25kD antigen which is found on rat T-cells. Does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.

Purification: Ammonium sulfate precipitation

Immunogen: F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.

Type of Immunogen: Cells

Isotype: IgM

Application Details

Application Notes: Approved: Flo (1:10 - 1:25), IHC, IHC-Fr (1:10 - 1:25), IHC-P (1:10), IP

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway