CD86 antibody

Catalog No. ABIN305514

This anti-CD86 antibody is a Mouse Monoclonal antibody detecting CD86 in FACS, WB, IHC, IP, IHC (fro), Func, BR and Neut. Suitable for Human.

Quick Overview for CD86 antibody (ABIN305514)

Target: CD86

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Blocking Reagent (BR), Neutralization (Neut)

Clone: BU63

Product Details anti-CD86 Antibody

Specificity: The antibody reacts with CD86, a 70 kD type I transmembrane gycoprotein of the immunoglobulin supergene family, expressed on antigen-presenting cells such as dendritic cells, macrophages or activated B lymphocytes.

Purification: Protein A purified

Immunogen: B-lymphoblastoid cell line ARH 77.

Isotype: IgG1

Application Details

Application Notes: Approved: Block, Flo, Func, IHC, IHC-Fr, IP, Neut, WB

Usage: Flow Cytometry: Recommended dilution - 5 μg/mL. Functional Application: The antibody BU63 blocks mixed lymphocyte reaction (MLR) and binding of soluble CTLA-4 (CD152)-mulg fusion protein to CD86 (B7-2).

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: Name/Gene ID: CD86

Synonyms: CD86, Activation B7-2 antigen, B7-2, B7.2, B70, CD86 Molecule, CTLA-4 counter-receptor B7.2, CD86 antigen, FUN-1, LAB72, BU63, CD28LG2, Ctla-4 counter-receptors b7.2

Gene ID: 942

UniProt: P42081

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation