CD86 antibody

Catalog No. ABIN305514

The Mouse Monoclonal anti-CD86 antibody (Clone BU63) (ABIN305514) specifically detects CD86 in FACS, WB, IHC, IP, IHC (fro), Func, BR and Neut. The antibody is reactive with Human samples.

Quick Overview for CD86 antibody (ABIN305514)

Target: CD86

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Blocking Reagent (BR), Neutralization (Neut)

Clone: BU63

Product Details anti-CD86 Antibody

Specificity: The antibody reacts with CD86, a 70 kD type I transmembrane gycoprotein of the immunoglobulin supergene family, expressed on antigen-presenting cells such as dendritic cells, macrophages or activated B lymphocytes.

Purification: Protein A purified

Immunogen: B-lymphoblastoid cell line ARH 77.

Isotype: IgG1

Application Details

Application Notes: Approved: Block, Flo, Func, IHC, IHC-Fr, IP, Neut, WB

Usage: Flow Cytometry: Recommended dilution - 5 μg/mL. Functional Application: The antibody BU63 blocks mixed lymphocyte reaction (MLR) and binding of soluble CTLA-4 (CD152)-mulg fusion protein to CD86 (B7-2).

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: Name/Gene ID: CD86

Synonyms: CD86, Activation B7-2 antigen, B7-2, B7.2, B70, CD86 Molecule, CTLA-4 counter-receptor B7.2, CD86 antigen, FUN-1, LAB72, BU63, CD28LG2, Ctla-4 counter-receptors b7.2

Gene ID: 942

UniProt: P42081

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation