IL20RA antibody (AA 159-175)

Catalog No. ABIN305719

This Rabbit Polyclonal antibody specifically detects IL20RA in WB, IHC, IHC (p) and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for IL20RA antibody (AA 159-175) (ABIN305719)

Target: IL20RA (Interleukin 20 Receptor alpha (IL20RA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL20RA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-IL20RA Antibody

Binding Specificity: AA 159-175

Brand: IHC-plus™

Specificity: The amino acid sequence used as immunogen is 100 % homologous in human and chimp, and 82 % homologous in mouse and rat. Both the expect ~70 kD band and an unknown ~90 kD band are seen in A375 cell lysate and are blocked by the immunizing peptide. The ~90 kD band alone is seen in A431 cell lysate, and it is also blocked by the immunizing peptide. This was made against a portion of the extracellular domain of IL-20RA. Permeabilization of the cells during testing may affect binding.

Purification: Protein G purified

Immunogen: Synthetic peptide from human IL20RA.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IL20RA

Target: IL20RA (Interleukin 20 Receptor alpha (IL20RA))

Alternative Name: IL20RA

Background: Name/Gene ID: IL20RA
Family: Interleukin

Synonyms: IL20RA, CRF2-8, IL-20 receptor subunit alpha, IL-20R1, IL-20R-alpha, Interleukin 20 receptor, alpha, Interleukin-20 receptor I, IL-20RA, ZCYTOR7

Gene ID: 53832

UniProt: Q9UHF4