Elastin antibody

Catalog No. ABIN305722

This Rabbit Polyclonal antibody specifically detects Elastin in IHC, ELISA, IHC (p), IF and RIA. It exhibits reactivity toward Human.

Quick Overview for Elastin antibody (ABIN305722)

Target: Elastin (ELN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Elastin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Product Details anti-Elastin Antibody

Brand: IHC-plus™

Specificity: Recognizes human Elastin. Crossreactivity: < 0.1 % with human Collagen I, II, III, IV, V, human Fibronectin, or rat Elastin by radioimmunoassay.

Purification: Protein A purified

Immunogen: Elastin extracted and purified from human skin

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA, IF (1:80), IHC, IHC-P (2.5 μg/mL), RIA

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Immunofluorescence: 1:80. Frozen human skin and liver tissues. Positive control: Human skin or liver.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS buffer,  pH 7.2 with no preservatives added

Preservative: Without preservative

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Elastin

Target: Elastin (ELN)

Alternative Name: ELN / Elastin

Background: Name/Gene ID: ELN

Synonyms: ELN, SVAS, WBS, Elastin, Tropoelastin, WS

Gene ID: 2006

UniProt: P15502

Pathways: Regulation of Actin Filament Polymerization