Adenosine A2a Receptor antibody (3rd Intracellular Domain)

Catalog No. ABIN305729

The Mouse Monoclonal anti-Adenosine A2a Receptor antibody has been validated for WB, IHC, FACS, IHC (p) and IHC (pfa). It is suitable to detect Adenosine A2a Receptor in samples from Human, Mouse, Rat, Dog, Rabbit and Guinea Pig.

Quick Overview for Adenosine A2a Receptor antibody (3rd Intracellular Domain) (ABIN305729)

Target: Adenosine A2a Receptor (ADORA2A)

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Adenosine A2a Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraformaldehyde) (IHC (pfa))

Clone: 7F6-G5-A2-7F6

Product Details anti-Adenosine A2a Receptor Antibody

Binding Specificity: 3rd Intracellular Domain

Brand: IHC-plus™

Specificity: Recognizes human adenosine receptor A2A at ~43kD.

Purification: Protein G purified

Immunogen: 6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (5 μg/mL), IHC-Pfa (1 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:1000-1:5000 detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M Tris-Glycine,  pH 7.4, 0.15M sodium chloride, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Alternative Name: ADORA2A/Adenosine A2A Receptor

Background: Name/Gene ID: ADORA2A
Subfamily: Adenosine
Family: GPCR

Synonyms: ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8

Gene ID: 135

UniProt: P29274

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints