Elastin antibody

Catalog No. ABIN305753

The Mouse Monoclonal anti-Elastin antibody has been validated for WB, IHC, IP, ELISA, IHC (p) and IF. It is suitable to detect Elastin in samples from Human, Mouse, Cow and Chicken.

Quick Overview for Elastin antibody (ABIN305753)

Target: Elastin (ELN)

Reactivity: Human, Mouse, Cow, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Elastin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: 10B8

Product Details anti-Elastin Antibody

Brand: IHC-plus™

Specificity: Recognizes chicken Elastin.

Purification: Protein A purified

Immunogen: Tropoelastin from 17-day old lathyric chick embryo aortae, extracted and purified according to the method of Rich and Foster, Meth. Enzymol. 82:665-673(1982).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Elastin

Target: Elastin (ELN)

Alternative Name: ELN / Elastin

Background: Name/Gene ID: ELN

Synonyms: ELN, SVAS, WBS, Elastin, Tropoelastin, WS

Gene ID: 2006

UniProt: P15502

Pathways: Regulation of Actin Filament Polymerization