GAP43 antibody

Catalog No. ABIN305762

The Mouse Monoclonal anti-GAP43 antibody has been validated for WB, IHC, ELISA, IHC (p) and IHC (fro). It is suitable to detect GAP43 in samples from Human, Rat, Mouse, Chicken, Snake and Rabbit.

Quick Overview for GAP43 antibody (ABIN305762)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivity: Human, Rat, Mouse, Chicken, Snake, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GAP43 Antibody

Brand: IHC-plus™

Specificity: Recognizes rat GAP-43. Reacts with both phosphorylated and unphosphorylated forms of the GAP-43 protein.

Purification: Protein A purified

Immunogen: Purified, full length GAP-43 protein (both phosphorylated & unphosphorylated forms).

Type of Immunogen: Purified protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-Fr (5 μg/mL), IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: Cell lysates derived from mouse and rat brain.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide. Sourced in TCS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: GAP43

Background: Name/Gene ID: GAP43
Family: Neuromodulin

Synonyms: GAP43, Axonal membrane protein GAP-43, B-50, Growth associated protein 43, Growth-associated protein 43, Neural phosphoprotein B-50, Neuromodulin, PP46, GAP-43, Protein F1

Gene ID: 2596

UniProt: P17677