Occludin antibody (C-Term)

Catalog No. ABIN305888

The Rabbit Polyclonal anti-Occludin antibody has been validated for WB, IHC, ELISA, IP, IF and IHC (p). It is suitable to detect Occludin in samples from Human, Mouse, Rat and Dog.

Quick Overview for Occludin antibody (C-Term) (ABIN305888)

Target: Occludin (OCLN)

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Occludin Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.

Purification: Immunoaffinity purified

Immunogen: A GST fusion protein consisting of the C-terminal 150 amino acids of human occludin fused to GST.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-P (2.5 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: OCLN / Occludin

Background: Name/Gene ID: OCLN

Synonyms: OCLN, Occludin, BLCPMG

Gene ID: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C