POLR2A/RPB1 antibody (pSer2, Ser5)

Catalog No. ABIN305952

This anti-POLR2A/RPB1 antibody is a Rabbit Polyclonal antibody detecting POLR2A/RPB1 in WB, IHC and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for POLR2A/RPB1 antibody (pSer2, Ser5) (ABIN305952)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POLR2A/RPB1 Antibody

Binding Specificity: pSer2, Ser5

Specificity: Detects endogenous levels of Rpb1 only when the carboxy-terminal domain heptapeptide repeat [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] is phosphorylated at serine 2/5. Species cross-reactivity: Human, mouse, and rat. Species sequence homology: Hamster.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser2/5 of the Rpb1 CTD heptapeptide.

Type of Immunogen: Synthetic phospho-peptide

Application Details

Application Notes: Approved: IHC, IHC-P (1:25), WB (1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:25 (Citrate/TBST).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A / RNA Polymerase II

Background: Name/Gene ID: POLR2A

Synonyms: POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1

Gene ID: 5430

UniProt: P24928

Pathways: Regulatory RNA Pathways