VAMP2 antibody (AA 1-89)

Catalog No. ABIN306417

Product Details anti-VAMP2 Antibody

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Binding Specificity: AA 1-89

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This VAMP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Purification: The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography

Immunogen: Recombinant human Synaptobrevin 2 (1-89aa) purified from E. coli

Clone: 3E5

Isotype: IgG1 kappa

Application Details

Application Notes: Recommended dilution 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In Phosphate-Buffered Saline (pH7.4) with 0.1% Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details for VAMP2

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Alternative Name: VAMP-2 (VAMP2 Products)

Background: Synaptobrevin 2 (Vesicle-associated membrane, VAMP2), which is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal tail. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25. This antibody recognizes specifically synaptobrevin 2, but it also shows a low affinity to synaptobrevin 1.

NCBI Accession: NP_055047

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport